9MOJ

Saccharolobus solfataricus GINS tetramer

Summary for 9MOJ

• Entry DOI: 10.2210/pdb9moj/pdb
• Descriptor: SsoGINS51, GINS subunit domain-containing protein (3 entities in total)
• Functional Keywords: gins, dna replication, replication
• Biological source: Saccharolobus solfataricus P2; More
• Total number of polymer chains: 4
• Total formula weight: 76970.16

Authors

Enemark, E.J.,Shankar, S. (deposition date: 2024-12-26, release date: 2025-04-23, Last modification date: 2025-05-07)

Primary citation

Shankar, S.,Enemark, E.J.
Structure of the Saccharolobus solfataricus GINS tetramer.
Acta Crystallogr.,Sect.F, 81:207-215, 2025

PubMed Abstract: DNA replication is tightly regulated to ensure genomic stability and prevent several diseases, including cancers. Eukaryotes and archaea partly achieve this regulation by strictly controlling the activation of hexameric minichromosome maintenance (MCM) helicase rings that unwind DNA during its replication. In eukaryotes, MCM activation critically relies on the sequential recruitment of the essential factors Cdc45 and a tetrameric GINS complex at the onset of the S-phase to generate a larger CMG complex. We present the crystal structure of the tetrameric GINS complex from the archaeal organism Saccharolobus solfataricus (Sso) to reveal a core structure that is highly similar to the previously determined GINS core structures of other eukaryotes and archaea. Using molecular modeling, we illustrate that a subdomain of SsoGINS would need to move to accommodate known interactions of the archaeal GINS complex and to generate a SsoCMG complex analogous to that of eukaryotes.

PubMed: 40235367
DOI: 10.1107/S2053230X25003085

Experimental method

X-RAY DIFFRACTION (2.3 Å)