9MOJ

Saccharolobus solfataricus GINS tetramer

9MOJ の概要

• エントリーDOI: 10.2210/pdb9moj/pdb
• 分子名称: SsoGINS51, GINS subunit domain-containing protein (3 entities in total)
• 機能のキーワード: gins, dna replication, replication
• 由来する生物種: Saccharolobus solfataricus P2; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 76970.16

構造登録者

Enemark, E.J.,Shankar, S. (登録日: 2024-12-26, 公開日: 2025-04-23, 最終更新日: 2025-05-07)

主引用文献

Shankar, S.,Enemark, E.J.
Structure of the Saccharolobus solfataricus GINS tetramer.
Acta Crystallogr.,Sect.F, 81:207-215, 2025

PubMed Abstract: DNA replication is tightly regulated to ensure genomic stability and prevent several diseases, including cancers. Eukaryotes and archaea partly achieve this regulation by strictly controlling the activation of hexameric minichromosome maintenance (MCM) helicase rings that unwind DNA during its replication. In eukaryotes, MCM activation critically relies on the sequential recruitment of the essential factors Cdc45 and a tetrameric GINS complex at the onset of the S-phase to generate a larger CMG complex. We present the crystal structure of the tetrameric GINS complex from the archaeal organism Saccharolobus solfataricus (Sso) to reveal a core structure that is highly similar to the previously determined GINS core structures of other eukaryotes and archaea. Using molecular modeling, we illustrate that a subdomain of SsoGINS would need to move to accommodate known interactions of the archaeal GINS complex and to generate a SsoCMG complex analogous to that of eukaryotes.

PubMed: 40235367
DOI: 10.1107/S2053230X25003085

実験手法

X-RAY DIFFRACTION (2.3 Å)