9MKX

FnoCas12a bridge helix variant state 4b

9MKX の概要

• エントリーDOI: 10.2210/pdb9mkx/pdb
• EMDBエントリー: 48341
• 分子名称: CRISPR-associated endonuclease Cas12a, RNA (31-MER), TS DNA, ... (4 entities in total)
• 機能のキーワード: cas12a, bridge helix, loop-to-helical transition, conformational cascade, allostery, off-target dna cleavage, dna binding protein
• 由来する生物種: Francisella tularensis subsp. novicida; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 180999.25

構造登録者

Ganguly, C.,Thomas, L.M.,Aribam, S.D.,Martin, L.,Rajan, R. (登録日: 2024-12-18, 公開日: 2026-02-11)

主引用文献

Ganguly, C.,Aribam, S.D.,Dos Santos, A.M.,Martin, L.,Thomas, L.M.,Shao, Y.,Rajan, R.
Bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation.
Nat Commun, 2026

PubMed Abstract: CRISPR-Cas12a, an RNA-based DNA targeting system, is widely used for genome editing and biomarker detection. To mitigate the off-target DNA cleavage of Cas12a, we previously developed a Francisella novicida Cas12a variant (FnoCas12a) by introducing double proline substitutions (K969P/D970P) in a conserved arginine-rich helix called the bridge helix (BH). In this work, we use a combinatorial approach to understand the molecular mechanisms of BH-mediated activation of Cas12a for DNA cleavage. We report five structures of FnoCas12a that are at different states of conformational activation. Comparison of the variant and wild-type (FnoCas12a) structures, along with activity assays and computational simulations, establishes the loop-to-helical transition and bending of the BH as an allosteric trigger for RNA-DNA hybrid propagation. These changes track with the previously reported coupled remodeling of BH and helix 1 of RuvC motif-II as well as the REC lobe movements needed to accommodate the growing hybrid. The transition of the BH is essential for the loop-to-helical transition of the "lid", which in turn opens the RuvC active site pocket for DNA entry and cleavage. Pairwise 3D structural comparison of the BH and RuvC of Cas12 and Cas9 families provides insight into the diversity of BH's structural organization in these mechanistically similar enzymes.

PubMed: 41605928
DOI: 10.1038/s41467-026-68657-0

実験手法

ELECTRON MICROSCOPY (3.21 Å)