Summary for 9MEE
| Entry DOI | 10.2210/pdb9mee/pdb |
| EMDB information | 48162 48163 48179 48214 |
| Descriptor | GOLLD RNA nanocage (1 entity in total) |
| Functional Keywords | golld, nanocage, ncrna, rna |
| Biological source | synthetic construct |
| Total number of polymer chains | 14 |
| Total formula weight | 3764450.82 |
| Authors | Kretsch, R.C.,Wu, Y.,Nye, G.,Das, R.,Chiu, W. (deposition date: 2024-12-06, release date: 2025-04-30, Last modification date: 2025-07-30) |
| Primary citation | Kretsch, R.C.,Wu, Y.,Shabalina, S.A.,Lee, H.,Nye, G.,Koonin, E.V.,Gao, A.,Chiu, W.,Das, R. Naturally ornate RNA-only complexes revealed by cryo-EM. Nature, 643:1135-1142, 2025 Cited by PubMed Abstract: The structures of natural RNAs remain poorly characterized and may hold numerous surprises. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryo-electron microscopy (cryo-EM). GOLLD (Giant, Ornate, Lake- and Lactobacillales-Derived), ROOL (Rumen-Originating, Ornate, Large) and OLE (Ornate Large Extremophilic) RNAs form homo-oligomeric complexes whose stoichiometries are retained at lower concentrations than measured in cells. OLE RNA forms a dimeric complex with long co-axial pipes spanning two monomers. Both GOLLD and ROOL form distinct RNA-only multimeric nanocages with diameters larger than the ribosome, each empty except for a disordered loop. Extensive intramolecular and intermolecular A-minor interactions, kissing loops, an unusual A-A helix and other interactions stabilize the three complexes. Sequence covariation analysis of these large RNAs reveals evolutionary conservation of intermolecular interactions, supporting the biological importance of large, ornate RNA quaternary structures that can assemble without any involvement of proteins. PubMed: 40328315DOI: 10.1038/s41586-025-09073-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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