9MEE

GOLLD RNA nanocage (env-38)

これはPDB形式変換不可エントリーです。

9MEE の概要

• エントリーDOI: 10.2210/pdb9mee/pdb
• EMDBエントリー: 48162 48163 48179 48214
• 分子名称: GOLLD RNA nanocage (1 entity in total)
• 機能のキーワード: golld, nanocage, ncrna, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 3764450.82

構造登録者

Kretsch, R.C.,Wu, Y.,Nye, G.,Das, R.,Chiu, W. (登録日: 2024-12-06, 公開日: 2025-04-30, 最終更新日: 2025-07-30)

主引用文献

Kretsch, R.C.,Wu, Y.,Shabalina, S.A.,Lee, H.,Nye, G.,Koonin, E.V.,Gao, A.,Chiu, W.,Das, R.
Naturally ornate RNA-only complexes revealed by cryo-EM.
Nature, 643:1135-1142, 2025

PubMed Abstract: The structures of natural RNAs remain poorly characterized and may hold numerous surprises. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryo-electron microscopy (cryo-EM). GOLLD (Giant, Ornate, Lake- and Lactobacillales-Derived), ROOL (Rumen-Originating, Ornate, Large) and OLE (Ornate Large Extremophilic) RNAs form homo-oligomeric complexes whose stoichiometries are retained at lower concentrations than measured in cells. OLE RNA forms a dimeric complex with long co-axial pipes spanning two monomers. Both GOLLD and ROOL form distinct RNA-only multimeric nanocages with diameters larger than the ribosome, each empty except for a disordered loop. Extensive intramolecular and intermolecular A-minor interactions, kissing loops, an unusual A-A helix and other interactions stabilize the three complexes. Sequence covariation analysis of these large RNAs reveals evolutionary conservation of intermolecular interactions, supporting the biological importance of large, ornate RNA quaternary structures that can assemble without any involvement of proteins.

PubMed: 40328315
DOI: 10.1038/s41586-025-09073-0

実験手法

ELECTRON MICROSCOPY (3 Å)