9MD9

Photoactivation in Bacteriophytochromes; 3ps TR-SFX structure

9MD9 の概要

• エントリーDOI: 10.2210/pdb9md9/pdb
• 分子名称: Photoreceptor-histidine kinase BphP, BILIVERDINE IX ALPHA, BENZAMIDINE, ... (4 entities in total)
• 機能のキーワード: phytochrome, sabphp2, time-resolved serial femtosecond crystallography, signaling protein
• 由来する生物種: Stigmatella aurantiaca
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 105248.46

構造登録者

Schmidt, M.,Malla, T. (登録日: 2024-12-05, 公開日: 2025-10-08)

主引用文献

Malla, T.N.,Aldama, L.,Leon, V.,Feliz, D.,Hu, H.,Thomas, I.,Cellini, A.,Wahlgren, W.Y.,Nimmrich, A.,Botha, S.,Sierra, R.,Hunter, M.S.,Poitevin, F.,Lisova, S.,Batyuk, A.,Gate, G.,Jernigan, R.,Kupitz, C.J.,Maj, P.,Meszaros, P.,Kurttila, M.,Monrroy, L.,Luo, F.,Owada, S.,Kang, J.,Slavov, C.,Maj, M.,Gautier, C.,Kashipathy, M.,Tolstikova, A.,Mariani, V.,Barty, A.,Moss, F.,Schwander, P.,Liu, H.,Boutet, S.,Fromme, P.,Takala, H.,Ihalainen, J.A.,Weierstall, U.,Westenhoff, S.,Stojkovic, E.A.,Schmidt, M.
Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.
Commun Chem, 8:183-183, 2025

PubMed Abstract: Myxobacteria are non-photosynthetic, soil-dwelling bacteria distinguished by a multicellular stage in their life cycle known as fruiting bodies that are stimulated by light. Myxobacterial phytochromes are candidates for the perception of red-light. The mechanism how light is perceived and converted to a physiological response is unknown. Here, time-resolved serial femtosecond crystallographic (TR-SFX) experiments were conducted on microcrystals of the photosensory core module of the Stigmatella aurantiaca bacteriophytochrome 2 (SaBphP2). Initial events of the Z to E isomerization reaction of the covalently bound, open-chain tetrapyrrole biliverdin (BV) chromophore were determined. At 3 ps after light activation, the BV ring-D assumes a configuration needed for the isomerization. At 100 ps, a mixture of BV in the Z or E configuration is observed in subunit A, while in the other subunit the chromophore remains in the Z configuration. In conjunction with prior results, these structures reveal the molecular mechanism of phytochrome activation in the photomorphogenesis of the myxobacteria and provide the molecular foundation for physiological responses to red light in other bacteria.

PubMed: 40506475
DOI: 10.1038/s42004-025-01578-z

実験手法

X-RAY DIFFRACTION (2.3 Å)