9MD6

Neuraminidase in complex with mAb 6-23.1

9MD6 の概要

• エントリーDOI: 10.2210/pdb9md6/pdb
• EMDBエントリー: 48169
• 分子名称: Neuraminidase, Heavy chain, Light chain, ... (6 entities in total)
• 機能のキーワード: polyclonal antibodies, influenza, neuraminidase, cryo-em, immune system, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 314870.38

構造登録者

Ferguson, J.A.,Raghavan, S.S.R.,Ward, A.B. (登録日: 2024-12-05, 公開日: 2025-08-27)

主引用文献

Ferguson, J.A.,Raghavan, S.S.R.,Alzua, G.P.,Bhavsar, D.,Huang, J.,Rodriguez, A.J.,Torres, J.L.,Bottermann, M.,Han, J.,Krammer, F.,Batista, F.D.,Ward, A.B.
Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryo-EM.
Sci Adv, 11:eadv8257-eadv8257, 2025

PubMed Abstract: Antibodies are crucial therapeutics, comprising a substantial portion of approved drugs due to their safety and clinical efficacy. Traditional antibody discovery methods are labor-intensive, limiting scalability and high-throughput analysis. Here, we improved upon our streamlined approach combining structural analysis and bioinformatics to infer heavy and light chain sequences from cryo-EM (cryo-electron microscopy) maps of serum-derived polyclonal antibodies (pAbs) bound to antigens. Using ModelAngelo, an automated structure-building tool, we accelerated pAb sequence determination and identified sequence matches in B cell repertoires via ModelAngelo-derived hidden Markov models (HMMs) associated with pAb structures. Benchmarking against results from a nonhuman primate HIV vaccine trial, our pipeline reduced analysis time from weeks to under a day with higher precision. Validation with murine immune sera from influenza vaccination revealed multiple protective antibodies. This workflow enhances antibody discovery, enabling faster, more accurate mapping of polyclonal responses with broad applications in vaccine development and therapeutic antibody discovery.

PubMed: 40815640
DOI: 10.1126/sciadv.adv8257

実験手法

ELECTRON MICROSCOPY (2.7 Å)