9LO2

Crystal Structure of Anti-CRISPR protein

9LO2 の概要

• エントリーDOI: 10.2210/pdb9lo2/pdb
• 分子名称: Anti-CRISPR protein, AcrIE7 (2 entities in total)
• 機能のキーワード: anti-crispr protein, viral protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 36043.82

構造登録者

Kang, J.,Bae, E.,Lee, G.,Koo, J.,Oh, H. (登録日: 2025-01-22, 公開日: 2025-06-18)

主引用文献

Kang, J.,Park, C.,Lee, G.,Koo, J.,Oh, H.,Kim, E.H.,Bae, E.,Suh, J.Y.
Structural Investigation of the Anti-CRISPR Protein AcrIE7.
Proteins, 2025

PubMed Abstract: The CRISPR-Cas system is an adaptive immune system in prokaryotes that provides protection against bacteriophages. As a countermeasure, bacteriophages have evolved various anti-CRISPR proteins that neutralize CRISPR-Cas immunity. Here, we report the structural and functional investigation of AcrIE7, which inhibits the type I-E CRISPR-Cas system in Pseudomonas aeruginosa. We determined both crystal and solution structures of AcrIE7, which revealed a novel helical fold. In binding assays using various biochemical methods, AcrIE7 did not tightly interact with a single Cas component in the type I-E Cascade complex or the CRISPR adaptation machinery. In contrast, AlphaFold modeling with our experimentally determined AcrIE7 structure predicted that AcrIE7 interacts with Cas3 in the type I-E CRISPR-Cas system in P. aeruginosa. Our findings are consistent with a model where AcrIE7 inhibits Cas3 and also highlight the effectiveness and limitations of AlphaFold modeling.

PubMed: 40318042
DOI: 10.1002/prot.26832

実験手法

X-RAY DIFFRACTION (2.287 Å)