9LII

structure of phage T4 topoisomerase II central domain

9LII の概要

• エントリーDOI: 10.2210/pdb9lii/pdb
• EMDBエントリー: 63118
• 分子名称: DNA topoisomerase medium subunit, DNA topoisomerase large subunit,DNA topoisomerase small subunit (2 entities in total)
• 機能のキーワード: topoisomerase ii, isomerase
• 由来する生物種: Enterobacteria phage T4 (Bacteriophage T4); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 258953.35

構造登録者

Chen, Y.T.,Xin, Y.H. (登録日: 2025-01-14, 公開日: 2025-11-26, 最終更新日: 2026-04-15)

主引用文献

Xin, Y.,Xian, R.,Liu, C.,Zhang, O.,Rao, Z.,Li, X.,Chen, Y.
Direct trapping of the transport-segment DNA by the central domain of type IIA topoisomerases.
Sci Adv, 11:eadw2839-eadw2839, 2025

PubMed Abstract: Type IIA topoisomerases modulate DNA topology by coordinating the cleavage of gate-segment DNA and the passage of transport-segment DNA-a mechanism conserved across species and essential for diverse cellular processes. While gate-segment interactions have been extensively studied, direct structural evidence of transport-segment capture has remained elusive, limiting our understanding of the full catalytic cycle. Here, we present a cryo-electron microscopy structure of the T4 bacteriophage topoisomerase II with a transport-segment DNA bound directly to its central domain. The structure reveals conformational rearrangements in the central domain that accommodate the transport-segment DNA, suggesting an alternative sequence of events in which the enzyme sliding along the loosely bound religated gate segment may precede transport-segment passage through the coiled-coil gate. Supported by mutational and biochemical assays, our findings provide previously unidentified mechanistic insights and open potential avenues for the development of next-generation type IIA topoisomerase inhibitors.

PubMed: 41406217
DOI: 10.1126/sciadv.adw2839

実験手法

ELECTRON MICROSCOPY (3.06 Å)