9LIB

Solution NMR structure of the bound antimicrobial peptide VR18 to Pseudomonas aeruginosa LPS bicelle at pH 4

9LIB の概要

• エントリーDOI: 10.2210/pdb9lib/pdb
• NMR情報: BMRB: 36724
• 分子名称: VR18 (VAL-ALA-ARG-GLY-TRP-GLY-ARG-LYS-CYS-PRO-LEU-PHE-GLY-LYS-ASN-LYS-SER-ARG) (1 entity in total)
• 機能のキーワード: structure from cyana 2.1, antimicrobial protein
• 由来する生物種: Dengue virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2066.48

構造登録者

Biswas, K.,Bhunia, A. (登録日: 2025-01-14, 公開日: 2025-09-17, 最終更新日: 2026-03-04)

主引用文献

Biswas, K.,Mitra, S.,Roy, D.,Roy, S.,Sarkar, D.,Son, D.,Das, R.,Roy, A.,Senapati, D.,Ilyas, H.,Harikishore, A.,Biswas, R.,Chakrabarty, S.,Lee, D.,Biswas, I.,Saha, S.,Kundu, P.,Bhunia, A.
Transgenic Tobacco Plants Expressing Synthetic Peptides: A Functional and Structural Analysis for Pathogen Resistance.
Plant Biotechnol J, 24:526-546, 2026

PubMed Abstract: The emergence of multidrug-resistant pathogens poses a serious threat to human health and agriculture. Current antimicrobial strategies against phytopathogens are often ineffective, failing to ensure food security while contributing to environmental pollution. Synthetic antimicrobial peptides (AMPs) offer a promising alternative due to their broad-spectrum activity and potential for recombinant production. In this study, we investigated the antibacterial potential of two synthetic peptides, VR18 and KG18, against animal as well as plant phytopathogens. Both peptides showed selective binding to bacterial membranes, while exhibiting no toxicity or allergenicity in animal cells. Using solution-state NMR, we explored how their structure relates to their function in disrupting bacterial membranes. When expressed transgenically in Nicotiana tabacum, VR18 and KG18 conferred resistance to Pseudomonas syringae pv. tabaci-a significant plant pathogen-without interfering with the plant's normal stress responses or metabolic activity. These results underscore the potential of AMPs as a sustainable, in vivo alternative to traditional antimicrobials in agriculture and open the door to broader applications in managing phytopathogenic threats.

PubMed: 40952904
DOI: 10.1111/pbi.70287

実験手法

SOLUTION NMR