9LEC

Focused asymmetric unit of Sag-18RS21 Golld RNA

9LEC の概要

• エントリーDOI: 10.2210/pdb9lec/pdb
• EMDBエントリー: 63022
• 分子名称: Sag-18RS21 Golld RNA (1 entity in total)
• 機能のキーワード: natural rna nanocages; large rna; cryo-em; golld, rna
• 由来する生物種: Streptococcus agalactiae 18RS21
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 122220.18

構造登録者

Zhang, K.,Li, S. (登録日: 2025-01-07, 公開日: 2025-09-10, 最終更新日: 2025-10-29)

主引用文献

Zhang, S.,Yi, R.,An, L.,Liu, J.,Yao, X.,Li, S.,Zhang, K.
Structural insights into higher-order natural RNA-only multimers.
Nat.Struct.Mol.Biol., 32:2012-2021, 2025

PubMed Abstract: RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in synthetic RNA nanomaterials, naturally occurring RNA-only complexes have primarily been observed as monomers or dimers, leaving higher-order assemblies largely unexplored. ROOL (rumen-originating, ornate, large) and GOLLD (giant, ornate, lake- and Lactobacillales-derived) RNAs are conserved non-coding RNAs with complex secondary structures, but their high-resolution architectures remain unknown. Here, we determine the cryo-electron microscopy structures of UCC118-Rool RNA, Sag-Golld RNA and Env38-Golld RNA at 1.96-2.98 Å resolution, revealing their distinct hexameric, decameric and tetradecameric assemblies. These higher-order architectures are stabilized by an array of tertiary motifs such as kissing loops and tetraloop-receptor motifs, underscoring the conserved principles of RNA self-assembly. By elucidating the molecular details of these higher-order RNA-only assemblies, this study expands our understanding of RNA-based architectures and broadens the scope of RNA structural biology.

PubMed: 40770114
DOI: 10.1038/s41594-025-01650-1

実験手法

ELECTRON MICROSCOPY (2.51 Å)