9L5Z

Crystal structure of a parallel-stranded PNA duplex

9L5Z の概要

• エントリーDOI: 10.2210/pdb9l5z/pdb
• 分子名称: (TPN)(GPN)(APN)(APN)(CPN)(TPN)(GPN)(CPN)(LYN), (APN)(CPN)(TPN)(TPN)(GPN)(APN)(CPN)(GPN)(LYN) (3 entities in total)
• 機能のキーワード: peptide nucleic acid, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 18578.63

構造登録者

Shibata, M.,Aiba, Y.,Sugimoto, H.,Shoji, O. (登録日: 2024-12-23, 公開日: 2025-12-10)

主引用文献

Shibata, M.,Sugimoto, H.,Hibino, M.,Shoji, O.,Aiba, Y.
Peptide nucleic acids in parallel orientation form invasion complexes with double-stranded DNA.
Rsc Chem Biol, 6:1566-1575, 2025

PubMed Abstract: Peptide nucleic acid (PNA) is a unique class of synthetic nucleic acids with a pseudo-peptide backbone, known for its high nucleic acid recognition capability and its ability to directly recognize double-stranded DNA (dsDNA) the formation of a unique invasion complex. While most natural and artificial nucleic acids form duplexes in an antiparallel configuration due to the general instability of parallel configurations, PNA distinctively forms both antiparallel and parallel duplexes. In this study, we focused on this previously underexplored property of PNA to adopt a parallel duplex configuration and developed a novel double-duplex invasion strategy by leveraging the differences in thermal stability between the antiparallel and parallel orientations of PNA duplexes. Furthermore, we report the first crystal structure of a parallel PNA duplex, which was found to exhibit different structural features compared to the previously characterized antiparallel PNA duplex. This study highlights the potential of artificial nucleic acids in dsDNA recognition and demonstrates that the parallel architecture may serve as a conceptual foundation for advancing broader methodological innovations in nucleic acid research.

PubMed: 40901609
DOI: 10.1039/d5cb00172b

実験手法

X-RAY DIFFRACTION (1.7 Å)