9KZU

Cryo-EM structure of the HCV IRES-dependently initiated CMV-stalled 80S ribosome (non-rotated state) in complexed with eIF3

これはPDB形式変換不可エントリーです。

9KZU の概要

• エントリーDOI: 10.2210/pdb9kzu/pdb
• 関連するPDBエントリー: 9KKF 9KN5 9KN6 9KZX
• EMDBエントリー: 62671
• 分子名称: 28S ribosomal RNA, 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (94 entities in total)
• 機能のキーワード: hcv ires, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 92
• 化学式量合計: 4523436.53

構造登録者

Iwasaki, W.,Kashiwagi, K.,Sakamoto, A.,Nishimoto, M.,Takahashi, M.,Machida, K.,Imataka, H.,Matsumoto, A.,Shichino, Y.,Iwasaki, S.,Imami, K.,Ito, T. (登録日: 2024-12-11, 公開日: 2025-12-03, 最終更新日: 2025-12-17)

主引用文献

Iwasaki, W.,Kashiwagi, K.,Sakamoto, A.,Nishimoto, M.,Takahashi, M.,Machida, K.,Imataka, H.,Matsumoto, A.,Shichino, Y.,Iwasaki, S.,Imami, K.,Ito, T.
Structural insights into the role of eIF3 in translation mediated by the HCV IRES.
Proc.Natl.Acad.Sci.USA, 122:e2505538122-e2505538122, 2025

PubMed Abstract: The genomes of various RNA viruses and a subset of human genes contain structured RNA elements termed internal ribosomal entry sites (IRESs) to initiate translation in a cap-independent manner. The well-studied IRES from Hepatitis C virus (HCV) binds to eukaryotic initiation factor 3 (eIF3), but how the HCV IRES harnesses eIF3 for viral translation remains unclear. Here, we determined multiple cryo-EM structures in which the HCV IRES binds simultaneously to the ribosome and eIF3, covering steps from initiation to elongation. The eIF3 core subunits are displaced from the ribosome by binding more tightly to subdomain IIIb of the HCV IRES. However, cross-linking mass spectrometry suggested that the eIF3 noncore subunits in the HCV-IRES-mediated elongation complex remain in similar positions on the ribosome to those observed in the cap-mediated initiation complex. This currently determined configuration of eIF3 core and noncore subunits reveals the mechanisms through which the HCV IRES overcomes the competition with the host mRNA and promotes viral mRNA translation by utilizing eIF3. Interestingly, cryo-EM structures also revealed that the N-terminal domain of the eIF3 c-subunit (eIF3c-NTD) binds to the large ribosomal subunit (60S) during elongation. These findings suggest that eIF3 contributes to HCV IRES-mediated translation not only during initiation but also elongation and potentially in reinitiation. The interaction between the eIF3c-NTD and the 60S ribosome is likely to occur in general translation processes as well, contributing to 60S joining or eIF3 stabilization on the elongating ribosome.

PubMed: 41337487
DOI: 10.1073/pnas.2505538122

実験手法

ELECTRON MICROSCOPY (3 Å)