9KWC

Cas12a-PCPS-light

9KWC の概要

• エントリーDOI: 10.2210/pdb9kwc/pdb
• EMDBエントリー: 62607
• 分子名称: LbCas12a, RNA (25-MER) (2 entities in total)
• 機能のキーワード: cas12a-pcps-dark, dna binding protein/rna, header dna binding protein-rna complex, header dna binding protein/rna
• 由来する生物種: Lachnospiraceae bacterium ND2006; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 151531.64

構造登録者

Zhang, M.F. (登録日: 2024-12-05, 公開日: 2025-07-16)

主引用文献

Hu, M.,Zhang, B.,Shan, Y.,Cao, F.,Wang, Y.,Qi, W.,Wang, X.,Shen, Y.,Guo, X.,Zhang, M.,Tian, T.,Xie, W.,Zhang, M.,Liang, F.,Pei, D.,Zhou, X.
Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA.
Nat Commun, 16:5939-5939, 2025

PubMed Abstract: The regulation of CRISPR‒Cas activity is critical for developing advanced biotechnologies. Optical control of CRISPR‒Cas system activity can be achieved by modulation of Cas proteins or guide RNA (gRNA), but these approaches either require complex protein engineering modifications or customization of the optically modulated gRNAs according to the target. Here, we present a method, termed photocleavable phosphorothioate DNA (PC&PS DNA)-mediated regulation of CRISPR‒Cas activity (DNACas), that is versatile and overcomes the limitations of conventional methods. In DNACas, CRISPR‒Cas activity is silenced by the affinity binding of PC&PS DNA and restored through light-triggered chemical bond breakage of PC&PS DNA. The universality of DNACas is demonstrated by adopting the PC&PS DNA to regulate various CRISPR‒Cas enzymes, achieving robust light-switching performance. DNACas is further adopted to develop a light-controlled one-pot LAMP-BrCas12b detection method and a spatiotemporal gene editing strategy. We anticipate that DNACas could be employed to drive various biotechnological advances.

PubMed: 40593724
DOI: 10.1038/s41467-025-61094-5

実験手法

ELECTRON MICROSCOPY (2.9 Å)