9KPR

Crystal structure of a allulose transcriptional regulator from Agrobacterium fabrum

これはPDB形式変換不可エントリーです。

9KPR の概要

• エントリーDOI: 10.2210/pdb9kpr/pdb
• 分子名称: Transcriptional regulator, LacI family, D-Allulose (3 entities in total)
• 機能のキーワード: catalysis, transcription
• 由来する生物種: Agrobacterium fabrum str. C58
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79369.91

構造登録者

Wei, H.L.,Dong, Q.Z.,Liu, W.D.,Yang, J.G.,Sun, Y.X. (登録日: 2024-11-24, 公開日: 2025-11-26, 最終更新日: 2026-01-14)

主引用文献

Dong, Q.,Chen, P.,Guo, Z.,Wei, H.,Zeng, Y.,Zhang, J.,Men, Y.,Liu, W.,Sun, Y.,Yang, J.
Computational design of allulose-responsive biosensor toolbox for auto-inducible protein expression and CRISPRi mediated dynamic metabolic regulation.
Nat Commun, 16:11562-11562, 2025

PubMed Abstract: Biosensors based on transcription factors (TFs) have shown extensive applications in synthetic biology. Due to the complex multi-domain structure of effector-TF-DNA, computational design of TFs remains a challenge. Here, we present the successful structure-guided computational design of the access tunnel, ligand binding, allosteric transition process for an allulose-responsive PsiR. It enables a 20-fold increase in sensitivity, reducing the EC of PsiR-allulose biosensors (PABs) from 16 mM to 0.8 mM, and delivers a PAB box possessing the detection range from 10 μM to 100 mM. We further validate its broader applicability in enhancing sensitivity of LacI-IPTG biosensor. Based on the developed PABs, we present the inducer-free allulose-mediated auto-inducible protein expression system, and demonstrate an allulose-triggered CRISPR interference circuit for dynamic metabolic regulation. It facilitates a 68% increase in allulose titer and achieves a high yield of 0.43 g/g glucose. This work provides the versatile TF toolbox for developing allulose-triggered regulation circuits in biotechnology application.

PubMed: 41430049
DOI: 10.1038/s41467-025-67669-6

実験手法

X-RAY DIFFRACTION (2.53 Å)