9KPJ

Crystal structure of Helicobacter pylori chemoreceptor TlpA ligand-binding domain in complex with indole

9KPJ の概要

• エントリーDOI: 10.2210/pdb9kpj/pdb
• 分子名称: Methyl-accepting chemotaxis protein TlpA, INDOLE, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: chemoreceptor, indole, helicobacter pylori, ligand-binding domain, signaling protein
• 由来する生物種: Helicobacter pylori PMSS1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33520.84

構造登録者

Wang, X.,Bi, S.Y. (登録日: 2024-11-22, 公開日: 2025-10-22, 最終更新日: 2025-11-05)

主引用文献

Wang, X.,Xu, W.,Yao, F.,Liu, S.,Zhang, Y.,Sourjik, V.,Bi, S.
Discovery of an indole-sensing chemoreceptor in Helicobacter pylori.
Curr.Biol., 35:5018-, 2025

PubMed Abstract: Indole has recently been recognized as a ubiquitous interspecies and interkingdom signaling molecule. However, the mechanisms underlying indole sensing in prokaryotes remain largely uncharacterized, and direct binding of indole to bacterial extracellular sensors has not been demonstrated. In this study, we show that the Helicobacter pylori chemoreceptor TlpA, which plays an important role in the host colonization by this gastric pathogen, binds indole and several related compounds, including menadione, through its dCache sensory domain. This binding occurs in the physiological range of indole concentrations and elicits a repellent response. The repellent response to indole mediated by the sensory domain of TlpA was also observed for the chimeric TlpA-Tar receptor in Escherichia coli. While ligands typically bind to the membrane-distal pocket of dCache domains, our structural and biochemical analyses demonstrate that indole binds to the membrane-proximal pocket of TlpA dCache. The TlpA-mediated negative chemotaxis of H. pylori correlates with the inhibitory effects of indole on bacterial growth and biofilm formation. The dCache domain of TlpA thus represents a new high-affinity sensory module for indole and related compounds that may be widespread in bacteria and play an important role in the physiology and pathogenicity of H. pylori.

PubMed: 41033314
DOI: 10.1016/j.cub.2025.09.021

実験手法

X-RAY DIFFRACTION (2.01 Å)