9KPH

Fusobacterium nucleatum ARRPOF RNA dimer conformation 2

9KPH の概要

• エントリーDOI: 10.2210/pdb9kph/pdb
• EMDBエントリー: 62487
• 分子名称: RNA (255-MER) (1 entity in total)
• 機能のキーワード: cryoem, rna
• 由来する生物種: Fusobacterium nucleatum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 164358.86

構造登録者

Tu, Y.F.,Shang, S.T.,Su, Z.M. (登録日: 2024-11-22, 公開日: 2025-03-19, 最終更新日: 2025-05-14)

主引用文献

Wang, L.,Xie, J.,Gong, T.,Wu, H.,Tu, Y.,Peng, X.,Shang, S.,Jia, X.,Ma, H.,Zou, J.,Xu, S.,Zheng, X.,Zhang, D.,Liu, Y.,Zhang, C.,Luo, Y.,Huang, Z.,Shao, B.,Ying, B.,Cheng, Y.,Guo, Y.,Lai, Y.,Huang, D.,Liu, J.,Wei, Y.,Sun, S.,Zhou, X.,Su, Z.
Cryo-EM reveals mechanisms of natural RNA multivalency.
Science, 388:545-550, 2025

PubMed Abstract: Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have rarely been reported. Comparative genomics analysis has identified RNA families containing hundreds of sequences that adopt conserved secondary structures and likely fold into complex three-dimensional structures. In this study, we used cryo-electron microscopy (cryo-EM) to determine structures from four RNA families, including ARRPOF and OLE forming dimers and ROOL and GOLLD forming hexameric, octameric, and dodecameric nanostructures, at 2.6- to 4.6-angstrom resolutions. These homo-oligomeric assemblies reveal a plethora of structural motifs that contribute to RNA multivalency, including kissing-loop, palindromic base-pairing, A-stacking, metal ion coordination, pseudoknot, and minor-groove interactions. These results provide the molecular basis of intermolecular interactions driving RNA multivalency with potential functional relevance.

PubMed: 40080543
DOI: 10.1126/science.adv3451

実験手法

ELECTRON MICROSCOPY (3.93 Å)