9KME

Crystal structure of soluble bacteriorhodopsin NeuroBR_A

9KME の概要

• エントリーDOI: 10.2210/pdb9kme/pdb
• 分子名称: soluble bacteriorhodopsin, RETINAL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: soluble bacteriorhodopsin, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 111619.16

構造登録者

Nikolaev, A.,Remeeva, A.,Kapranov, I.,Borshchevskiy, V.,Gushchin, I. (登録日: 2024-11-15, 公開日: 2025-06-04, 最終更新日: 2025-07-02)

主引用文献

Nikolaev, A.,Orlov, Y.,Tsybrov, F.,Kuznetsova, E.,Shishkin, P.,Kuzmin, A.,Mikhailov, A.,Nikolaeva, Y.S.,Anuchina, A.,Chizhov, I.,Semenov, O.,Kapranov, I.,Borshchevskiy, V.,Remeeva, A.,Gushchin, I.
Engineering of soluble bacteriorhodopsin.
Chem Sci, 16:11067-11076, 2025

PubMed Abstract: Studies and applications of membrane proteins remain challenging due to the requirement of maintaining them in a lipid membrane or a membrane mimic. Modern machine learning-based protein engineering methods offer a possibility of generating soluble analogs of membrane proteins that retain the active site structure and ligand-binding properties; however, clear examples are currently missing. Here, we report successful engineering of proteins dubbed NeuroBRs that mimic the active site (retinal-binding pocket) of bacteriorhodopsin, a light-driven proton pump and well-studied model membrane protein. NeuroBRs are soluble and stable, bind retinal and exhibit photocycles under illumination. The crystallographic structure of NeuroBR_A, determined at anisotropic resolution reaching 1.76 Å, reveals an excellently conserved chromophore binding pocket and tertiary structure. Thus, NeuroBRs are promising microbial rhodopsin mimics for studying retinal photochemistry and potential soluble effector modules for optogenetic tools. Overall, our results highlight the power of modern protein engineering approaches and pave the way towards wider development of molecular tools derived from membrane proteins.

PubMed: 40406218
DOI: 10.1039/d5sc02453f

実験手法

X-RAY DIFFRACTION (1.76 Å)