9KMA

Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana

9KMA の概要

• エントリーDOI: 10.2210/pdb9kma/pdb
• 分子名称: Polynucleotide adenylyltransferase family protein (1 entity in total)
• 機能のキーワード: cca-adding enzyme, trna processing, protein sorting, rna binding protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66100.99

構造登録者

Wang, X.,Li, Y.Y.,Dou, Z.Y.,Liu, L. (登録日: 2024-11-15, 公開日: 2025-06-18, 最終更新日: 2025-07-02)

主引用文献

Wang, X.,Li, Y.Y.,Dou, Z.Y.,Wang, J.,Liu, L.
Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana .
J Struct Biol X, 11:100127-100127, 2025

PubMed Abstract: The 3'-terminal CCA-end of tRNA is essential for the attachment of amino acids and correct positioning of the aminoacyl-tRNA in the ribosome. In higher plants, the CCA sequence is synthesized, maintained, and repaired by class-II CCA-adding enzymes encoded by a single nuclear gene but multi-targeted to the nucleus, cytoplasm, plastids, and mitochondria. The structure of plant class-II CCA-adding enzyme remains unsolved. Here we describe the crystal structure of CCA-adding enzyme from (CCA) The overall structure of CCA is similar to other class-II CCA-adding enzymes but significant differences occur in the body domain. Structural comparison of body and tail domains between CCA and other class-II CCA-adding enzymes unravels three specific regions of CCA. Based on the modeled CCA-tRNA complex, CCA may have a different tRNA binding pattern. The three specific regions located in the body domain of CCA also provide candidate regions for multi-targeted sorting.

PubMed: 40519583
DOI: 10.1016/j.yjsbx.2025.100127

実験手法

X-RAY DIFFRACTION (3.229 Å)