9KLD

Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 2) with covalently bound D-ring

9KLD の概要

• エントリーDOI: 10.2210/pdb9kld/pdb
• 関連するPDBエントリー: 9KKX
• EMDBエントリー: 62397 62401
• 分子名称: SKP1-like protein 1A, Hyposensitive to light 7, F-box protein, ... (5 entities in total)
• 機能のキーワード: strigolactone, scf, ubiquitination, signaling protein
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 250725.95

構造登録者

Vancea, A.I.,Huntington, B.,Savva, C.G.,Arold, S.T. (登録日: 2024-11-14, 公開日: 2025-09-17, 最終更新日: 2025-12-03)

主引用文献

Vancea, A.I.,Huntington, B.,Steinchen, W.,Savva, C.G.,Shahul Hameed, U.F.,Arold, S.T.
Mechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex.
Nat Commun, 16:10291-10291, 2025

PubMed Abstract: Strigolactones are plant hormones that regulate development and mediate interactions with soil organisms, including the germination of parasitic plants such as Striga hermonthica. Strigolactone perception by receptors initiates the degradation of transcriptional repressors via E3 ubiquitin ligases, but the mechanistic link between hormone binding and substrate ubiquitination has remained unclear. We determine cryogenic electron microscopy structures of the receptor-ligase-substrate complex, composed of Arabidopsis ASK1 and substrate, and Striga F-box and receptor proteins. Strigolactone hydrolysis by the receptor, which covalently retains the D-ring, is a prerequisite for complex formation. The substrate engages the complex through two domains, forming a dynamic interface that stabilises the receptor-ligase assembly and repositions the ASK1, suggesting a mechanism for efficient ubiquitination. Here, we show how dynamic, multivalent interactions within the receptor-ligase-substrate complex translate hormone perception into targeted protein degradation, providing insight into how plants integrate hormonal signals into developmental decisions.

PubMed: 41271672
DOI: 10.1038/s41467-025-65205-0

実験手法

ELECTRON MICROSCOPY (2.9 Å)