9K3N

The structure of Salmonella phage PJNS002

これはPDB形式変換不可エントリーです。

9K3N の概要

• エントリーDOI: 10.2210/pdb9k3n/pdb
• EMDBエントリー: 62023
• 分子名称: capsid protein F, spike protein G, DNA-binding protein J, ... (5 entities in total)
• 機能のキーワード: structual proteins, virus
• 由来する生物種: Salmonella phage PJNS002; 詳細
• タンパク質・核酸の鎖数: 300
• 化学式量合計: 4376472.84

構造登録者

Hu, W.L.,Chen, Y.B.,Wei, Y.M.,Gao, Y. (登録日: 2024-10-19, 公開日: 2025-08-13, 最終更新日: 2025-08-20)

主引用文献

Hu, W.,Liu, Z.,Wei, Y.,Bian, Q.,Lan, W.,Fan, C.,Song, J.,Sun, Q.,Zhang, X.,Liu, Y.,Gao, Y.,Chen, Y.
Structural basis for Salmonella infection by two Microviridae phages.
Commun Biol, 8:1166-1166, 2025

PubMed Abstract: The global resurgence of multidrug-resistant Salmonella species, responsible for millions of annual infections, underscores the urgent need for alternative antimicrobial strategies, such as phage therapy. Microviridae phages offer a promising model for studying phage-host interactions with their unique structural and infection mechanisms. Here, we identify two Microviridae phages, PJNS001 and PJNS002, with different host receptor dependencies, and determine their cryo-EM structures at 2.68 Å and 2.59 Å resolution, respectively. These icosahedral capsids with T = 1 symmetry exhibit a unique vertex reinforcement mechanism, stabilizing the viral assembly. The specific pentameric adaptations, coupled with DNA binding protein engagements and thermodynamic constraints, collectively preclude the formation of hybrid virions. Structural analysis and in situ visualization reveal spike protein features and host-attachment intermediates, informing host specificity. Together, these findings advance our understanding of Microviridae infection mechanisms and provide a structural framework for rational phage design against antibiotic-resistant pathogens.

PubMed: 40770068
DOI: 10.1038/s42003-025-08595-7

実験手法

ELECTRON MICROSCOPY (2.59 Å)