9K09 の概要
| エントリーDOI | 10.2210/pdb9k09/pdb |
| EMDBエントリー | 61942 |
| 分子名称 | Tail fiber protein, Portal protein, Tail tubular protein B, ... (4 entities in total) |
| 機能のキーワード | cyanophage, virus, tail, portal, zozzle, adaptor, tail fiber, viral protein |
| 由来する生物種 | Anabaena phage A-4L 詳細 |
| タンパク質・核酸の鎖数 | 48 |
| 化学式量合計 | 2586518.96 |
| 構造登録者 | |
| 主引用文献 | Hou, P.,Zhou, R.Q.,Jiang, Y.L.,Yu, R.C.,Du, K.,Gan, N.,Ke, F.,Zhang, Q.Y.,Li, Q.,Zhou, C.Z. Cryo-EM structure of cyanopodophage A4 reveals a pentameric pre-ejectosome in the double-stabilized capsid. Proc.Natl.Acad.Sci.USA, 122:e2423403122-e2423403122, 2025 Cited by PubMed Abstract: Upon infection, the podophages usually eject a couple of proteins from the capsid to form a transmembrane ejectosome on the host cell membrane that facilitates the ejection of viral genome. However, it remains unclear how these proteins of pre-ejectosome are finely assembled at the center of highly packaged genome. Here, we report the intact structure of cyanopodophage A4, which consists of a capsid stabilized by two types of cement proteins and a short tail attached with six tail fibers. Notably, we find a pentameric pre-ejectosome at the core of capsid, which is composed of four ejection proteins wrapped into a coaxial cylinder of triple layers. Moreover, a segment of genomic DNA runs along the positively charged circular cleft formed by two ejection proteins. Based on the mortise-and-tenon architecture of pre-ejectosome in combination with previous studies, we propose a putative DNA packaging process and ejection mechanism for podophages. These findings largely enrich our knowledge on the assembly mechanism of podophages, which might facilitate the application of A4 as a chassis cyanophage in synthetic biology. PubMed: 40163721DOI: 10.1073/pnas.2423403122 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (2.6 Å) |
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