9JX2
Structure of rsCherry exposed to oxygen for 8 days
Summary for 9JX2
| Entry DOI | 10.2210/pdb9jx2/pdb |
| Related | 8B65 8B7G |
| Descriptor | Red fluorescent protein drFP583, DI(HYDROXYETHYL)ETHER (3 entities in total) |
| Functional Keywords | red fluorescent protein, reversibly switching, degradation, fluorescent protein |
| Biological source | Discosoma sp. (Sea anemone) |
| Total number of polymer chains | 1 |
| Total formula weight | 31311.07 |
| Authors | |
| Primary citation | Bui, T.Y.H.,Pecqueur, L.,Dedecker, P.,Van Meervelt, L. Crystal structure analysis of oxygen-induced degradation occurring in rsCherry. Acta Crystallogr.,Sect.F, 81:311-318, 2025 Cited by PubMed Abstract: rsCherry was one of the first reversibly photoswitchable variants to be developed from mCherry. However, its practical applications have been limited due to several inherent drawbacks. We have recently shown that the purified protein undergoes oxygen-induced chromophore degradation in solution, resulting in the progressive loss of its fluorescence and color. In this work, we present four crystal structures of rsCherry that exhibit varying degrees of degradation. Our structural analysis indicates that oxygen-induced degradation of rsCherry predominantly affects the chromophore without altering the protein backbone. Changes were only observed in the conformation of Lys70, confirming the crucial role of this residue in chromophore damage in rsCherry. Overall, this study provides valuable insights into the structural changes triggered by oxygen exposure in rsCherry, offering suggestions for the development of stable red fluorescent proteins with improved resistance to oxidative damage. PubMed: 40552439DOI: 10.1107/S2053230X25005485 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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