9JX2

Structure of rsCherry exposed to oxygen for 8 days

9JX2 の概要

• エントリーDOI: 10.2210/pdb9jx2/pdb
• 関連するPDBエントリー: 8B65 8B7G
• 分子名称: Red fluorescent protein drFP583, DI(HYDROXYETHYL)ETHER (3 entities in total)
• 機能のキーワード: red fluorescent protein, reversibly switching, degradation, fluorescent protein
• 由来する生物種: Discosoma sp. (Sea anemone)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31311.07

構造登録者

Bui, T.Y.H.,Van Meervelt, L. (登録日: 2024-10-11, 公開日: 2025-07-09, 最終更新日: 2026-03-18)

主引用文献

Bui, T.Y.H.,Pecqueur, L.,Dedecker, P.,Van Meervelt, L.
Crystal structure analysis of oxygen-induced degradation occurring in rsCherry.
Acta Crystallogr.,Sect.F, 81:311-318, 2025

PubMed Abstract: rsCherry was one of the first reversibly photoswitchable variants to be developed from mCherry. However, its practical applications have been limited due to several inherent drawbacks. We have recently shown that the purified protein undergoes oxygen-induced chromophore degradation in solution, resulting in the progressive loss of its fluorescence and color. In this work, we present four crystal structures of rsCherry that exhibit varying degrees of degradation. Our structural analysis indicates that oxygen-induced degradation of rsCherry predominantly affects the chromophore without altering the protein backbone. Changes were only observed in the conformation of Lys70, confirming the crucial role of this residue in chromophore damage in rsCherry. Overall, this study provides valuable insights into the structural changes triggered by oxygen exposure in rsCherry, offering suggestions for the development of stable red fluorescent proteins with improved resistance to oxidative damage.

PubMed: 40552439
DOI: 10.1107/S2053230X25005485

実験手法

X-RAY DIFFRACTION (2 Å)