9JQK

Ebola VP30 and its inhibitor

これはPDB形式変換不可エントリーです。

9JQK の概要

• エントリーDOI: 10.2210/pdb9jqk/pdb
• 分子名称: Transcriptional activator VP30, 2-[(~{E})-heptadec-10-enyl]-6-oxidanyl-benzoic acid, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: vp30, viral protein
• 由来する生物種: Ebola virus (ZEBOV, Zaire Ebola virus)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 74042.44

構造登録者

Wu, F.,Xu, W. (登録日: 2024-09-27, 公開日: 2025-04-23)

主引用文献

Peng, C.,Wu, F.,Ma, Y.,Liu, G.,Huang, Y.,Tong, R.,Xu, W.
Ginkgolic acid inhibits Ebola virus transcription and replication by disrupting the interaction between nucleoprotein and VP30 protein.
Antiviral Res., 234:106074-106074, 2025

PubMed Abstract: The Ebola virus, a filovirus, has been responsible for significant human fatalities since its discovery. Despite extensive research, effective small-molecule drugs remain elusive due to its complex pathogenesis. Inhibition of RNA synthesis is a promising therapeutic target, and the VP30 protein plays a critical role in this process. The interaction between VP30 and the nucleoprotein (NP) is essential for viral replication. We identified ginkgolic acid as a small molecule with strong affinity for VP30, which was validated through multiple assays, including thermal shift, surface plasmon resonance, fluorescence polarization, pull-down, and co-immunoprecipitation. The antiviral efficacy of ginkgolic acid was demonstrated in the EBOV transcription- and replication-competent virus-like particle (trVLP) system. Furthermore, we resolved the crystal structure of the VP30-ginkgolic acid complex, revealing two ginkgolic acid molecules located at the VP30/NP interaction interface. This structural information provides insight into the molecular basis of ginkgolic acid's antiviral activity and suggests a novel therapeutic strategy targeting the VP30/NP interaction.

PubMed: 39716669
DOI: 10.1016/j.antiviral.2024.106074

実験手法

X-RAY DIFFRACTION (2.29 Å)