9JPP

Structure of the Bacterial Ribosome with human tRNA Lys(mcm5s2U34) and mRNA(AAG)

これはPDB形式変換不可エントリーです。

9JPP の概要

• エントリーDOI: 10.2210/pdb9jpp/pdb
• 関連するPDBエントリー: 9JPM 9JPO 9JQ2
• EMDBエントリー: 61706 61708 61709 61720
• 分子名称: 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (57 entities in total)
• 機能のキーワード: trna modifications, decoding, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 55
• 化学式量合計: 2212261.95

構造登録者

Ishiguro, K.,Mo, Y.,Shirouzu, M.,Suzuki, T. (登録日: 2024-09-26, 公開日: 2026-03-11, 最終更新日: 2026-07-01)

主引用文献

Mo, Y.,Ishiguro, K.,Miyauchi, K.,Sakaguchi, Y.,Hanzawa, Y.,Akiyama, N.,Murayama, A.,Machida, K.,Imataka, H.,Yamashita, A.,Ohira, T.,Shirouzu, M.,Suzuki, T.
Translational regulation by oxidative desulfuration of tRNA modifications.
Nat Commun, 17:-, 2026

PubMed Abstract: Modifications in the anticodon region of transfer RNA (tRNA) are essential for accurate and efficient protein synthesis. 5-Methyl-2-thiouridine derivatives (xmsU) are major modifications at the wobble position of tRNA anticodons decoding purine-ending two-codon sets. Although the thiocarbonyl group of xmsU enhances decoding efficiency, it is chemically susceptible to oxidative desulfuration, yielding 4-pyrimidinone derivatives (xmhU). Here, we identify xmhU derivatives in human cells and mouse tissues and confirm their cellular formation by spike-in experiments. Desulfurized tRNAs carrying 5-methoxycarbonylmethyl-4-pyrimidinone (mcmhU) show impaired codon recognition in a human reconstituted in vitro translation system. The mcmhU modification reduces aminoacylation of tRNAs for lysine, glutamate, and glutamine, but not arginine. Cryogenic electron microscopy reveals the structural basis of altered AAA/AAG decoding by mcmhU at the ribosomal A-site. These findings reveal a mechanism by which oxidative desulfuration of tRNA modifications dynamically regulates codon recognition and protein synthesis under oxidative stress conditions in human and mammalian cells.

PubMed: 41807381
DOI: 10.1038/s41467-026-70126-7

実験手法

ELECTRON MICROSCOPY (2.26 Å)