9JNP

Structure of isw1-nucleosome complex in ATP state

9JNP の概要

• エントリーDOI: 10.2210/pdb9jnp/pdb
• EMDBエントリー: 61624
• 分子名称: Histone H3, Histone H4, Histone H2A, ... (9 entities in total)
• 機能のキーワード: chromatin remodeler, nucleosome, dna binding protein/dna, dna binding protein-dna complex
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 321973.72

構造登録者

Sia, Y.,Pan, H.,Chen, Z. (登録日: 2024-09-23, 公開日: 2025-04-09, 最終更新日: 2025-06-18)

主引用文献

Sia, Y.,Pan, H.,Chen, K.,Chen, Z.
Structural insights into chromatin remodeling by ISWI during active ATP hydrolysis.
Science, 388:eadu5654-eadu5654, 2025

PubMed Abstract: Chromatin remodelers utilize the energy of adenosine triphosphate (ATP) hydrolysis to slide nucleosomes, regulating chromatin structure and gene activity in cells. In this work, we report structures of imitation switch (ISWI) bound to the nucleosome during active ATP hydrolysis and remodeling, revealing conformational transitions of the remodeling motor across the adenosine triphosphatase (ATPase) cycle. The DNA strands are distorted accordingly, showing one full base-pair bulge and a loss of histone contact at the site of motor binding in the adenosine diphosphate* b and Apo* states. We also identify several important elements for regulation of the remodeling activity. Notably, an enzyme conformation exiting the remodeling cycle reveals a linker DNA-sensing brake mechanism. Together, our findings elucidate a multistate model of ISWI action, providing a comprehensive mechanism of DNA translocation and regulation underpinning chromatin remodeling.

PubMed: 40179160
DOI: 10.1126/science.adu5654

実験手法

ELECTRON MICROSCOPY (2.3 Å)