9JKO

Crystal structure of the RNA- binding domain of human ZAR1

Summary for 9JKO

• Entry DOI: 10.2210/pdb9jko/pdb
• Descriptor: Zygote arrest protein 1, ZINC ION (3 entities in total)
• Functional Keywords: rna, zar1, rna binding protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 24033.83

Authors

Liu, Q.L.,Xu, C. (deposition date: 2024-09-16, release date: 2025-07-30)

Primary citation

Liu, Q.,Ma, H.,He, X.,Xu, C.,Zhang, J.
Molecular insights into RNA recognition by the ZAR1 C-terminal domain.
Febs Lett., 2025

PubMed Abstract: Zygote arrested-1 (ZAR1)-dependent translational repression plays an important role during early oogenesis. Here, we solved the crystal structure of the C-terminal domain of ZAR1, which contains three zinc-binding motifs, and confirmed its ability to bind to RNAs derived from translation control sequence elements within the 3'-UTRs of Wee1 and Mos mRNAs. By using the AlphaFold server, we obtained a predicted model for the structure of the ZAR1 C-terminal domain bound with a 13-nt RNA. Mutagenesis and biochemistry experiments further validated the ZAR1-RNA interaction. Therefore, our study provides insights into RNA recognition by the ZAR1 zinc-binding domain and the role of ZAR1 in repressing gene expression. Impact statement Zygote arrested-1 (ZAR1)-dependent translational repression finely orchestrates gene expression during early oogenesis. Here, we solved the high-resolution structure of the C-terminal zinc-binding domain of ZAR1 and confirmed its binding to RNA. The modeled ZAR1-RNA complex by Alphafold further provides insight into RNA recognition by ZAR1.

PubMed: 40388637
DOI: 10.1002/1873-3468.70080

Experimental method

X-RAY DIFFRACTION (1.63 Å)