9JKG

Asymmetric structure of cleaved HIV-1 Tri FPPR envelope glycoprotein trimer in amphipol-lipid nanodiscs (Tri FPPR.2)

これはPDB形式変換不可エントリーです。

9JKG の概要

• エントリーDOI: 10.2210/pdb9jkg/pdb
• EMDBエントリー: 61554
• 分子名称: Envelope glycoprotein gp160, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (14 entities in total)
• 機能のキーワード: viral entry, viral protein
• 由来する生物種: Simian-Human immunodeficiency virus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 538707.74

構造登録者

Qi, Y.,Zhang, S.,Sodroski, J.,Mao, Y. (登録日: 2024-09-16, 公開日: 2025-02-26, 最終更新日: 2025-04-02)

主引用文献

Qi, Y.,Zhang, S.,Wang, K.,Ding, H.,Zhang, Z.,Anang, S.,Nguyen, H.T.,Kappes, J.C.,Sodroski, J.,Mao, Y.
The membrane-proximal external region of human immunodeficiency virus (HIV-1) envelope glycoprotein trimers in A18-lipid nanodiscs.
Commun Biol, 8:442-442, 2025

PubMed Abstract: During human immunodeficiency virus (HIV-1) entry, the metastable pretriggered envelope glycoprotein (Env) trimer ((gp120/gp41)) opens asymmetrically. We present cryo-EM structures of cleaved asymmetric Env trimers in amphipol-lipid nanodiscs. The gp41 membrane-proximal external region (MPER) could be traced in Env protomers that remained close to the nanodisc despite Env tilting. The MPER interacts with the gp120 C-termini and gp41 α9 helices at the base of the Env trimer. MPER conformation is coupled with the tilt angles of the α9 helices, the helicity of the gp41 heptad repeat (HR1) regions, and the opening angles between the protomers of the asymmetric trimers. Our structural models explain the stabilizing effects of MPER integrity and Env proteolytic maturation on the pretriggered Env conformation. Superimposed on the asymmetry of the Env protomers, variation in the glycans at the trimer apex creates substantial structural heterogeneity in the V2 quaternary epitopes of difficult-to-elicit broadly neutralizing antibodies.

PubMed: 40089599
DOI: 10.1038/s42003-025-07852-z

実験手法

ELECTRON MICROSCOPY (3.5 Å)