9JHT

Jumbo phage Killer B

Summary for 9JHT

• Entry DOI: 10.2210/pdb9jht/pdb
• Descriptor: JukB, ZINC ION (3 entities in total)
• Functional Keywords: tetramer, immune system, antiviral protein
• Biological source: Shewanella xiamenensis
• Total number of polymer chains: 2
• Total formula weight: 74881.54

Authors

Wang, H.,Feng, Y.,Cao, X.L.,Guan, L.L. (deposition date: 2024-09-10, release date: 2025-04-16, Last modification date: 2025-04-30)

Primary citation

Yuping, L.,Guan, L.,Becher, I.,Makarova, K.S.,Cao, X.,Hareendranath, S.,Guan, J.,Stein, F.,Yang, S.,Boergel, A.,Lapouge, K.,Remans, K.,Agard, D.,Savitski, M.,Typas, A.,Koonin, E.V.,Feng, Y.,Bondy-Denomy, J.
Jumbo phage killer immune system targets early infection of nucleus-forming phages.
Cell, 188:2127-2140.e21, 2025

PubMed Abstract: Jumbo bacteriophages of the ϕKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify "jumbo phage killer" (Juk), a two-component immune system that terminates infection of ϕKZ-like phages, suppressing the expression of early phage genes and preventing phage DNA replication and phage nucleus assembly while saving the cell. JukA (formerly YaaW) rapidly senses the EPI vesicle by binding to an early-expressed phage protein, gp241, and then directly recruits JukB. The JukB effector structurally resembles a pore-forming toxin and destabilizes the EPI vesicle. Functional anti-ϕKZ JukA homologs are found across bacterial phyla, associated with diverse effectors. These findings reveal a widespread defense system that specifically targets early events executed by ϕKZ-like jumbo phages prior to phage nucleus assembly.

PubMed: 40112800
DOI: 10.1016/j.cell.2025.02.016

Experimental method

X-RAY DIFFRACTION (2.44 Å)