9JHT

Jumbo phage Killer B

9JHT の概要

• エントリーDOI: 10.2210/pdb9jht/pdb
• 分子名称: JukB, ZINC ION (3 entities in total)
• 機能のキーワード: tetramer, immune system, antiviral protein
• 由来する生物種: Shewanella xiamenensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74881.54

構造登録者

Wang, H.,Feng, Y.,Cao, X.L.,Guan, L.L. (登録日: 2024-09-10, 公開日: 2025-04-16, 最終更新日: 2025-04-30)

主引用文献

Yuping, L.,Guan, L.,Becher, I.,Makarova, K.S.,Cao, X.,Hareendranath, S.,Guan, J.,Stein, F.,Yang, S.,Boergel, A.,Lapouge, K.,Remans, K.,Agard, D.,Savitski, M.,Typas, A.,Koonin, E.V.,Feng, Y.,Bondy-Denomy, J.
Jumbo phage killer immune system targets early infection of nucleus-forming phages.
Cell, 188:2127-2140.e21, 2025

PubMed Abstract: Jumbo bacteriophages of the ϕKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify "jumbo phage killer" (Juk), a two-component immune system that terminates infection of ϕKZ-like phages, suppressing the expression of early phage genes and preventing phage DNA replication and phage nucleus assembly while saving the cell. JukA (formerly YaaW) rapidly senses the EPI vesicle by binding to an early-expressed phage protein, gp241, and then directly recruits JukB. The JukB effector structurally resembles a pore-forming toxin and destabilizes the EPI vesicle. Functional anti-ϕKZ JukA homologs are found across bacterial phyla, associated with diverse effectors. These findings reveal a widespread defense system that specifically targets early events executed by ϕKZ-like jumbo phages prior to phage nucleus assembly.

PubMed: 40112800
DOI: 10.1016/j.cell.2025.02.016

実験手法

X-RAY DIFFRACTION (2.44 Å)