9JFP

Structure of LaTranC complex bound to 6nt complementary DNA substrate

9JFP の概要

• エントリーDOI: 10.2210/pdb9jfp/pdb
• EMDBエントリー: 61435
• 分子名称: DNA (37-MER), RNA (195-MER), LaTranC, ... (4 entities in total)
• 機能のキーワード: latranc complexes, immune system/dna/rna, immune system-dna-rna complex
• 由来する生物種: Lawsonibacter sp.; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 139682.12

構造登録者

Zhang, S.,Liu, J. (登録日: 2024-09-05, 公開日: 2025-10-08, 最終更新日: 2025-11-26)

主引用文献

Jin, S.,Zhu, Z.,Li, Y.,Zhang, S.,Liu, Y.,Li, D.,Li, Y.,Luo, Y.,Cheng, Z.,Zhao, K.T.,Gao, Q.,Yang, G.,Li, H.,Liang, R.,Zhang, R.,Qiu, J.L.,Zhang, Y.E.,Liu, J.G.,Gao, C.
Functional RNA splitting drove the evolutionary emergence of type V CRISPR-Cas systems from transposons.
Cell, 188:6283-6300.e22, 2025

PubMed Abstract: Transposon-encoded TnpB nucleases gave rise to type V CRISPR-Cas12 effectors through multiple independent domestication events. These systems use different RNA molecules as guides for DNA targeting: transposon-derived right-end RNAs (reRNAs or omega RNAs) for TnpB and CRISPR RNAs for type V CRISPR-Cas systems. However, the molecular mechanisms bridging transposon activity and CRISPR immunity remain unclear. We identify TranCs (transposon-CRISPR intermediates) derived from distinct IS605- or IS607-TnpB lineages. TranCs utilize both CRISPR RNAs and reRNAs to direct DNA cleavage. The cryoelectron microscopy (cryo-EM) structure of LaTranC from Lawsonibacter sp. closely resembles that of the ISDra2 TnpB complex; however, unlike a single-molecule reRNA, the LaTranC guide RNA is functionally split into a tracrRNA and crRNA. An engineered RNA split of ISDra2 TnpB enabled activity with a CRISPR array. These findings indicate that functional RNA splitting was the primary molecular event driving the emergence of diverse type V CRISPR-Cas systems from transposons.

PubMed: 41027434
DOI: 10.1016/j.cell.2025.09.004

実験手法

ELECTRON MICROSCOPY (2.96 Å)