9JCN

Crystal structure of Zea mays 3-phosphoglycerate dehydrogenase S282L mutant

9JCN の概要

• エントリーDOI: 10.2210/pdb9jcn/pdb
• 分子名称: D-3-phosphoglycerate dehydrogenase (1 entity in total)
• 機能のキーワード: dehydrogenase, catabolism, catalysis, oxidoreductases, oxidoreductase
• 由来する生物種: Zea mays
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79183.00

構造登録者

Li, R.,Wang, C. (登録日: 2024-08-30, 公開日: 2025-07-16)

主引用文献

Zhang, Y.,Li, R.,Zheng, D.,Zhao, J.,Qing, K.,He, R.,Ma, Z.,Chen, J.,Xue, N.,Tian, X.,Wang, E.,Xu, J.,Li, Y.,Tan, B.C.,Zhou, Z.,Wang, C.,Dong, J.
Phosphoglycerate dehydrogenase is required for kernel development and defines a predominant serine synthesis pathway in maize.
Plant Cell, 37:-, 2025

PubMed Abstract: Serine functions as both a substrate for protein biosynthesis and a signaling molecule for growth and development. However, the mechanism remains poorly understood. Here, we cloned and functionally characterized the maize (Zea mays) gene Dek20, which encodes phosphoglycerate dehydrogenase1 (PGDH1), the rate-limiting enzyme in the phosphorylated pathway of serine biosynthesis (PPSB). The dek20(Ser282Leu) mutation disrupts the interaction between residues Ser282 and His284, leading to the release of His284, which subsequently binds NAD+/NADH to inhibit serine biosynthesis. Consequently, serine content decreases dramatically, and the cellular response to nutrient starvation is enriched in transcriptome analysis. Serine deficiency triggers tRNASer degradation and reduced translation elongation at serine codons. The stalled ribosomes activate General Control Nonderepressible 2 (GCN2) kinase, which affects the phosphorylation of eukaryotic initiation factor 2α (eIF2α) and ribosomal protein S6 kinase (S6 K), furtherly inhibiting translation initiation. Consistent with these findings, polysome profiling and Ribo-seq analysis revealed a marked decrease in translation efficiency in dek20. Notably, proteins essential for storage compound biosynthesis and cell cycle progression exhibit reduced translation in dek20. Collectively, our findings reveal the primary serine biosynthesis pathway and a mechanism for monitoring amino acid levels in maize, the model plant with C4 photosynthesis.

PubMed: 40403140
DOI: 10.1093/plcell/koaf126

実験手法

X-RAY DIFFRACTION (3.48 Å)