9JC7

Crystal structure of alanyl-tRNA synthetase in complex with ATP and L-alanine

9JC7 の概要

• エントリーDOI: 10.2210/pdb9jc7/pdb
• 分子名称: Alanine--tRNA ligase, ADENOSINE-5'-TRIPHOSPHATE, ALANINE, ... (8 entities in total)
• 機能のキーワード: ligase
• 由来する生物種: Methylomonas sp. DH-1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99995.42

構造登録者

Son, S.Y.,Cha, S.S. (登録日: 2024-08-28, 公開日: 2025-06-18, 最終更新日: 2025-06-25)

主引用文献

Park, W.,Son, S.Y.,Yi, J.,Cha, S.,Moon, H.,Kim, M.,Ji, S.,Yu, W.,Sung, C.,Cha, S.S.,Hahn, J.S.
Unlocking the serine mischarging paradox and inhibiting lactyltransferase activity of AlaRS by a single-point mutation.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Aminoacyl-tRNA synthetases are critical for accurate genetic translation, attaching amino acids to their corresponding transfer RNA molecules. Alanyl-tRNA synthetase (AlaRS) often misactivates Ser or Gly instead of Ala, which is detrimental unless corrected by its editing functions. The paradox of misactivating larger Ser by AlaRS was considered inevitable due to its inherent design, sharing an essential acidic residue to accommodate the activated adenylated intermediates from both cognate and non-cognate amino acids. Here we show a groundbreaking discovery where a single-point mutation, L219M, in AlaRS from Methylomonas sp. DH-1, effectively eliminates Ser misactivation. Structural analysis of the pre-activation state unveiled that the flexibility of Val204 is the key to preventing Ser binding in AlaRSL219M. This research elucidates the amino acid discrimination mechanism in AlaRS, independent of editing domain. Remarkably, the AlaRSL219M mutation was initially identified as a causal mutation enhancing lactate tolerance in a strain developed through adaptive laboratory evolution. We showed that AlaRSL219M also eliminates the enzyme's inherent lactyltransferase activity, suggesting that the lactate tolerance observed might result from preventing excessive protein lactylation under lactate stress. This opens possibilities for developing high-fidelity and lactylation-deficient AlaRS mutants across various organisms, facilitating studies on their potential benefits in different physiological scenarios.

PubMed: 40479712
DOI: 10.1093/nar/gkaf462

実験手法

X-RAY DIFFRACTION (1.9000034846 Å)