9JB5

Crystal structure of Arabidopsis theliana FRATAXIN HOMOLOG

Summary for 9JB5

• Entry DOI: 10.2210/pdb9jb5/pdb
• Descriptor: Frataxin, mitochondrial (2 entities in total)
• Functional Keywords: arabidopsis, metal-binding, iron transport, heme biosynthesis, transport protein
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 2
• Total formula weight: 30453.83

Authors

Zhang, J.,Wang, J.,Liu, L. (deposition date: 2024-08-26, release date: 2025-01-01, Last modification date: 2025-03-19)

Primary citation

Zhang, J.,Zhou, Y.,Duan, Q.,Xu, X.,Wang, X.,Wang, J.,Liu, L.
Characterization of Arabidopsis thaliana FRATAXIN HOMOLOG in heme catabolism.
Biochimie, 231:110-115, 2025

PubMed Abstract: Frataxin plays vital roles in various iron related processes. Arabidopsis thaliana FRATAXIN HOMOLOG (AtFH) is the first identified plant frataxin and has been found to regulate the last step of heme biosynthesis. Here, we report the involvement of AtFH in heme catabolism by regulating the activity of heme oxygenase. AtFH forms a homodimer, and its crystal structure shows the dimeric interactions. A structural comparison with known frataxin structures suggests the iron binding sites, and the site for heme oxygenase activity is possibly located in a region containing Glu78. The results indicate a previously uncharacterized role of plant frataxin in heme catabolism.

PubMed: 39722354
DOI: 10.1016/j.biochi.2024.12.010

Experimental method

X-RAY DIFFRACTION (2.8 Å)