9J48 の概要
| エントリーDOI | 10.2210/pdb9j48/pdb |
| EMDBエントリー | 61130 |
| 分子名称 | Designed ankyrin repeat proteins,Ferritin heavy chain, N-terminally processed, Green fluorescent protein (2 entities in total) |
| 機能のキーワード | gfp, darpin, apoferritin, scaffold, metal binding protein/luminescent protein, metal binding protein-luminescent protein complex |
| 由来する生物種 | Homo sapiens (human) 詳細 |
| タンパク質・核酸の鎖数 | 48 |
| 化学式量合計 | 1677420.48 |
| 構造登録者 | |
| 主引用文献 | Lu, X.,Yan, M.,Cai, Y.,Song, X.,Chen, H.,Du, M.,Wang, Z.,Li, J.,Niu, L.,Zeng, F.,Hao, Q.,Zhang, H. A large, general and modular DARPin-apoferritin scaffold enables the visualization of small proteins by cryo-EM. Iucrj, 12:393-402, 2025 Cited by PubMed Abstract: Single-particle cryo-electron microscopy (cryo-EM) has emerged as an indispensable technique in structural biology that is pivotal for deciphering protein architectures. However, the medium-sized proteins (30-40 kDa) that are prevalent in both eukaryotic and prokaryotic organisms often elude the resolving capabilities of contemporary cryo-EM methods. To address this challenge, we engineered a scaffold strategy that securely anchors proteins of interest to a robust, symmetric base via a selective adapter. Our most efficacious constructs, namely models 4 and 6c, feature a designed ankyrin-repeat protein (DARPin) rigidly linked to an octahedral human apoferritin via a helical linker. By utilizing these large, highly symmetric scaffolds (∼1 MDa), we achieved near-atomic-resolution cryo-EM structures of green fluorescent protein (GFP) and maltose-binding protein (MBP), revealing nearly all side-chain densities of GFP and the distinct structural features of MBP. The modular design of our scaffold allows the adaptation of new DARPins through minor amino-acid-sequence modifications, enabling the binding and visualization of a diverse array of proteins. The high symmetry and near-spherical shape of the scaffold not only mitigates the prevalent challenge of preferred particle orientation in cryo-EM but also significantly reduces the demands of image collection and data processing. This approach presents a versatile solution, breaking through the size constraints that have traditionally limited single-particle cryo-EM. PubMed: 40277178DOI: 10.1107/S2052252525003021 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.04 Å) |
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