9IW1

wild type NMN/NaMN adenylyltransferase from Chaetomium thermophilum

9IW1 の概要

• エントリーDOI: 10.2210/pdb9iw1/pdb
• 分子名称: Nicotinamide-nucleotide adenylyltransferase, BETA-NICOTINAMIDE RIBOSE MONOPHOSPHATE, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: adenylyltransferase, complex, transferase
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75439.05

構造登録者

Qian, X.-L.,Zheng, Y.-C.,Chen, C.,Xu, J.-H. (登録日: 2024-07-24, 公開日: 2025-06-25)

主引用文献

Qian, X.L.,Li, J.Y.,Li, C.X.,Pan, J.,Mu, B.,Xu, J.H.
Biochemical and structural characterization of a novel nicotinamide mononucleotide adenylyltransferase from thermophilic fungi Chaetomium thermophilum.
Biochem.Biophys.Res.Commun., 776:152192-152192, 2025

PubMed Abstract: Nicotinamide mononucleotide adenylyltransferases (NMNAT EC: 2.7.7.1) play pivotal roles in synthesis of nicotinamide adenine dinucleotide (NAD) through catalyzing the reaction of nicotinamide mononucleotide (NMN) with adenosine triphosphate (ATP). To date, multiple crystal structures of NMNAT originated from bacteria, archaea, and eukaryote have been resolved. However, none structure of NMNAT from thermophilic fungi was elucidated. Here we report the structure of nicotinamide mononucleotide adenylyltransferase (CtNMNAT) from thermophilic fungi Chaetomium thermophilum at a resolution of 2.10 Å. The enzyme crystals were in space group of P222 with two monomers per asymmetric unit. A nicotinamide mononucleotide (NMN) molecule was identified in the active pocket. Enzyme activity assays confirmed that CtNMNAT has a relatively high activity and good thermostability. The pH and temperature optima of CtNMNAT were pH 6.0 and 60 °C. CtNMNAT was stable at 50 °C with a half-life of 25.9 h. The specificity constant k/K of CtNMNAT toward NMN was 180 s mM 28.5 mM NAD was produced when using CtNMNAT as catalyst with a space-time yield of 72.0 g L d. This work provides a starting point for further investigation into the role of CtNMNAT in the NAD  metabolism of Chaetomium thermophilum and useful guidance for industrial application of CtNMNAT in the biocatalytic synthesis of NAD.

PubMed: 40516445
DOI: 10.1016/j.bbrc.2025.152192

実験手法

X-RAY DIFFRACTION (2.11 Å)