9IS4

Cryo-EM structure of a TEF30-associated intermediate C2S-type PSII-LHCII supercomplex from Chlamydomonas reinhardtii

Summary for 9IS4

• Entry DOI: 10.2210/pdb9is4/pdb
• EMDB information: 60830
• Descriptor: PDZ domain-containing protein, Photosystem II reaction center protein I, Photosystem II reaction center protein K, ... (39 entities in total)
• Functional Keywords: photosystem ii, psii repair, psii assembly, psii intermediate, tef30, c2s, supercomplex, photosynthesis
• Biological source: Chlamydomonas reinhardtii; More
• Total number of polymer chains: 38
• Total formula weight: 809235.15

Authors

Wang, Y.,Wang, C.,Li, A.,Liu, Z. (deposition date: 2024-07-16, release date: 2025-05-21, Last modification date: 2025-07-30)

Primary citation

Wang, Y.,Wang, C.,Li, A.,Liu, Z.
Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii.
Nat.Plants, 11:1455-1469, 2025

PubMed Abstract: During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a sophisticated biological process assisted by numerous auxiliary proteins. Here we report the cryogenic electron microscopy structures of four PSII-repair complexes from Chlamydomonas reinhardtii associated with the Thylakoid Enriched Fraction 30 (TEF30, an orthologue of plant MET1) protein-namely, a TEF30-PSII core monomer (TEF30-C), two types of TEF30-PSII core dimers (types I and II, TEF30-C-I and TEF30-C-II) and a TEF30-CS-type PSII-LHCII supercomplex (TEF30-CS; S, strongly associated light-harvesting complex II trimer). TEF30 mediates the assembly of CP43 with the RC47 module by clamping on the stromal surfaces and prevents the premature association of peripheral antennae with PSII-C. In the transition from TEF30-C-I to TEF30-C-II, TEF30-CS and mature CS, one PSII core slides along the dimerization interface against the adjacent one by 22-35 Å, generating a zigzagged surface for accommodating the peripheral antennae. These results suggest that the PSII repair process undergoes multiple TEF30-mediated intermediate states to form intact PSII-LHCII supercomplexes.

PubMed: 40579438
DOI: 10.1038/s41477-025-02036-3

Experimental method

ELECTRON MICROSCOPY (2.9 Å)