9IMP

The complex of PDZ3 and PBM

これはPDB形式変換不可エントリーです。

9IMP の概要

• エントリーDOI: 10.2210/pdb9imp/pdb
• 分子名称: Partitioning defective 3 homolog, INSC spindle orientation adaptor protein (3 entities in total)
• 機能のキーワード: complex, protein, peptide binding protein
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 78526.42

構造登録者

Huang, S.J. (登録日: 2024-07-04, 公開日: 2024-09-11, 最終更新日: 2024-09-18)

主引用文献

Huang, S.,Fu, M.,Gu, A.,Zhao, R.,Liu, Z.,Hua, W.,Mao, Y.,Wen, W.
mInsc coordinates Par3 and NuMA condensates for assembly of the spindle orientation machinery in asymmetric cell division.
Int.J.Biol.Macromol., 279:135126-135126, 2024

PubMed Abstract: As a fundamental process governing the self-renewal and differentiation of stem cells, asymmetric cell division is controlled by several conserved regulators, including the polarity protein Par3 and the microtubule-associated protein NuMA, which orchestrate the assembly and interplay of the Par3/Par6/mInsc/LGN complex at the apical cortex and the LGN/Gαi/NuMA/Dynein complex at the mitotic spindle to ensure asymmetric segregation of cell fate determinants. However, this model, which is well-supported by genetic studies, has been challenged by evidence of competitive interaction between NuMA and mInsc for LGN. Here, the solved crystal structure of the Par3/mInsc complex reveals that mInsc competes with Par6β for Par3, raising questions about how proteins assemble overlapping targets into functional macromolecular complexes. Unanticipatedly, we discover that Par3 can recruit both Par6β and mInsc by forming a dynamic condensate through phase separation. Similarly, the phase-separated NuMA condensate enables the coexistence of competitive NuMA and mInsc with LGN in the same compartment. Bridge by mInsc, Par3/Par6β and LGN/NuMA condensates coacervate, robustly enriching all five proteins both in vitro and within cells. These findings highlight the pivotal role of protein condensates in assembling multi-component signalosomes that incorporate competitive protein-protein interaction pairs, effectively overcoming stoichiometric constraints encountered in conventional protein complexes.

PubMed: 39218187
DOI: 10.1016/j.ijbiomac.2024.135126

実験手法

X-RAY DIFFRACTION (2.87 Å)