9ILL

monomeric SarA-E89Q in complex with DNA

9ILL の概要

• エントリーDOI: 10.2210/pdb9ill/pdb
• 分子名称: Transcriptional regulator SarA, DNA (2 entities in total)
• 機能のキーワード: winged-helix protein, complex structure, dna binding protein
• 由来する生物種: Staphylococcus aureus subsp. aureus N315; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 24826.98

構造登録者

Xia, B.,Fu, D.H. (登録日: 2024-06-30, 公開日: 2025-10-15)

主引用文献

Xia, B.,Fu, D.H.
solution Structure of the monomeric SarA in complex with DNA
Biomol.NMR Assign, 17:193-197, 2023

PubMed Abstract: SarA is a global transcription regulator in S. aureus which regulates the expression of over 120 genes related to quorum sensing, biofilm synthesis, drug resistance and many other important physiological processes during host infection. SarA can bind to the promoter region of agr and other target genes to activate or repress the transcription. The crystal structure of SarA uncovered a MarR protein-like conformation with two symmetrical winged helix domains, while its DNA binding mechanism is still unknown. We have constructed a monomeric DNA binding domain of SarA (SarA) for the study of the interaction between SarA and DNA with NMR spectroscopy. Here, we report the H, C and N NMR assignment of SarA/DNA complex which is the first step towards further structure and function analysis.

PubMed: 37405582
DOI: 10.1007/s12104-023-10140-8

実験手法

SOLUTION NMR