9IJM

Bacterial flagellar sodium-driven stator PomA5PomB2 with 100 mM NaCl and 0.1 mM phenamil

これはPDB形式変換不可エントリーです。

9IJM の概要

• エントリーDOI: 10.2210/pdb9ijm/pdb
• EMDBエントリー: 60636
• 分子名称: PomB, Chemotaxis protein PomA, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: bacterial flagellar stator unit, phenamil, sodium ion transport, membrane protein complex, membrane protein
• 由来する生物種: Vibrio alginolyticus; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 209426.93

構造登録者

Nishikino, T.,Takekawa, N.,Imada, K. (登録日: 2024-06-24, 公開日: 2025-01-01, 最終更新日: 2025-01-22)

主引用文献

Nishikino, T.,Takekawa, N.,Kishikawa, J.I.,Hirose, M.,Kojima, S.,Homma, M.,Kato, T.,Imada, K.
Structural insight into sodium ion pathway in the bacterial flagellar stator from marine Vibrio.
Proc.Natl.Acad.Sci.USA, 122:e2415713122-e2415713122, 2025

PubMed Abstract: Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ion flow to mechanical rotation. However, the ion pathway and the mechanism of stator rotation coupled with specific ion flow are still obscure. Here, we determined the structures of the sodium-driven stator of , namely PomAB, in the presence and absence of sodium ions and the structure with its specific inhibitor, phenamil, by cryo-electron microscopy. The structures and following functional analysis revealed the sodium ion pathway, the mechanism of ion selectivity, and the inhibition mechanism by phenamil. We propose a model of sodium ion flow coupled with the stator rotation based on the structures. This work provides insights into the molecular mechanisms of ion specificity and conversion of the electrochemical potential into mechanical functions.

PubMed: 39793043
DOI: 10.1073/pnas.2415713122

実験手法

ELECTRON MICROSCOPY (3.32 Å)