9IHC

CryoEM structure of a synthetic antibody, COP-2, in complex with the C-terminal domain of Clostridium perfringens Enterotoxin

9IHC の概要

• エントリーDOI: 10.2210/pdb9ihc/pdb
• EMDBエントリー: 52864
• 分子名称: Heat-labile enterotoxin B chain, COP-2 antibody heavy chain, COP-2 antibody light chain (3 entities in total)
• 機能のキーワード: clostridium, cop-2, toxin
• 由来する生物種: Clostridium perfringens; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 68967.16

構造登録者

Pacesa, M.,Goldbach, N.,Vecchio, A.J.,Correia, B.E. (登録日: 2025-02-21, 公開日: 2025-03-12, 最終更新日: 2025-09-24)

主引用文献

Ogbu, C.P.,Goldbach, N.M.,Pacesa, M.,Kapoor, S.,Correia, B.E.,Vecchio, A.J.
Structures of a synthetic antibody selected against and bound to the C-terminal domain of Clostridium perfringens enterotoxin.
Protein Sci., 34:e70281-e70281, 2025

PubMed Abstract: Clostridium perfringens enterotoxin (CpE) causes cytotoxic gastrointestinal disease in mammalian epithelium by binding membrane protein receptors called claudins. Claudins direct the formation of cell/cell tight junctions through oligomerization and govern the transport of molecules between individual cells. CpE binds claudins through its C-terminal domain (cCpE) and induces cytotoxicity through its N-terminal domain. The non-toxic cCpE is a useful tool to study claudins, tight junctions, and for translational applications, such as increasing the permeability of restrictive tissues like the blood-brain barrier or selective targeting of claudin overexpressing cancers. Conversely, there are no specialized molecular tools to study CpE or cCpE, or to modulate or inhibit their functions. We previously reported the development of synthetic antigen-binding fragments (sFabs) that bind cCpE, and low-resolution structures of them bound to claudin/cCpE complexes. Here, we determine high-resolution structures of sFab COP-2 bound to cCpE using X-ray crystallography and cryogenic electron microscopy. The structures and biophysical findings provide the mechanism of COP-2 binding to cCpE and the molecular determinants driving their interactions. These insights can advance the design of new antibody-based tools from our COP-2 scaffold to study or alter cCpE function and give rise to a "Trojan horse" strategy that exploits cCpE's tight junction barrier disrupting function to selectively deliver conjugated therapeutics through normally impermeable tissues.

PubMed: 40944397
DOI: 10.1002/pro.70281

実験手法

ELECTRON MICROSCOPY (2.95 Å)