9IGN

Aerolysin E254A/E258A in styrene-maleic acid lipid particles

これはPDB形式変換不可エントリーです。

9IGN の概要

• エントリーDOI: 10.2210/pdb9ign/pdb
• 関連するPDBエントリー: 9fm6 9fmL
• EMDBエントリー: 52853
• 分子名称: Aerolysin (1 entity in total)
• 機能のキーワード: pore forming toxin styrene maleic acid lipid particle, toxin
• 由来する生物種: Aeromonas hydrophila
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 658967.20

構造登録者

Anton, J.S.,Bada Juarez, J.F.,Marcaida, M.J.,Dal Peraro, M. (登録日: 2025-02-19, 公開日: 2025-11-26, 最終更新日: 2026-01-28)

主引用文献

Mayer, S.F.,Mitsioni, M.F.,Robin, P.,van den Heuvel, L.,Ronceray, N.,Marcaida, M.J.,Abriata, L.A.,Krapp, L.F.,Anton, J.S.,Soussou, S.,Jeanneret-Grosjean, J.,Fulciniti, A.,Moller, A.,Vacle, S.,Feletti, L.,Brinkerhoff, H.,Laszlo, A.H.,Gundlach, J.H.,Emmerich, T.,Dal Peraro, M.,Radenovic, A.
Lumen charge governs gated ion transport in beta-barrel nanopores.
Nat Nanotechnol, 21:116-124, 2026

PubMed Abstract: β-Barrel nanopores are involved in crucial biological processes, from ATP export in mitochondria to bacterial resistance, and represent a promising platform for emerging sequencing technologies. However, in contrast to ion channels, the understanding of the fundamental principles governing ion transport through these nanopores remains largely unexplored. Here we integrate experimental, numerical and theoretical approaches to elucidate ion transport mechanisms in β-barrel nanopores. We identify and characterize two distinct nonlinear phenomena: open-pore rectification and gating. Through extensive mutation analysis of aerolysin nanopores, we demonstrate that open-pore rectification is caused by ionic accumulation driven by the distribution of lumen charges. In addition, we provide converging evidence suggesting that gating is controlled by electric fields dissociating counterions from lumen charges, promoting local structural deformations. Our findings establish a rigorous framework for characterizing and understanding ion transport processes in protein-based nanopores, enabling the design of adaptable nanofluidic biotechnologies. We illustrate this by optimizing an aerolysin mutant for computing applications.

PubMed: 41219410
DOI: 10.1038/s41565-025-02052-6

実験手法

ELECTRON MICROSCOPY (2.3 Å)