9I53

Crystal structure of the SARS-CoV-2 helicase NSP13 in complex with ATP

9I53 の概要

• エントリーDOI: 10.2210/pdb9i53/pdb
• 関連するPDBエントリー: 9I51
• 分子名称: SARS-CoV-2 helicase NSP13, ZINC ION, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: nsp13, helicase, sars-cov-2, hydrolase
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 136915.05

構造登録者

Kloskowski, P.,Neumann, P.,Ficner, R. (登録日: 2025-01-27, 公開日: 2025-07-23, 最終更新日: 2025-08-13)

主引用文献

Kloskowski, P.,Neumann, P.,Berndt, A.,Ficner, R.
Nucleotide-bound crystal structures of the SARS-CoV-2 helicase NSP13.
Acta Crystallogr.,Sect.F, 81:338-347, 2025

PubMed Abstract: Nucleotide-bound crystal structures of SARS-CoV-2 NSP13 in ADP- and ATP-bound states were resolved to 1.8 and 1.9 Å, respectively. The ADP-bound model captures a state immediately following ATP hydrolysis, with both ADP and orthophosphate still present in the active site. Further comparative analysis revealed that crystal packing influences NSP13 by stabilizing the nucleotide-binding site, underscoring the importance of accounting for these effects in structure-based drug design targeting NSP13.

PubMed: 40638074
DOI: 10.1107/S2053230X25005266

実験手法

X-RAY DIFFRACTION (1.92 Å)