9HTC

McCP in complex with photocaged nitric oxide, 1.44 s, 1600 microjoule, SSX

9HTC の概要

• エントリーDOI: 10.2210/pdb9htc/pdb
• 分子名称: Cytochrome c, HEME C, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: mccp, heme, gas binding, no, metal binding protein
• 由来する生物種: Methylococcus capsulatus str. Bath
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36353.32

構造登録者

Smyth, P.,Williams, L.J.,Hough, M.A.,Worrall, J.A.R.,Owen, R.L. (登録日: 2024-12-19, 公開日: 2025-09-03, 最終更新日: 2025-09-10)

主引用文献

Smyth, P.,Jaho, S.,Williams, L.J.,Karras, G.,Fitzpatrick, A.,Thompson, A.J.,Battah, S.,Axford, D.,Horrell, S.,Lucic, M.,Ishihara, K.,Kataoka, M.,Matsuura, H.,Shimba, K.,Tono, K.,Tosha, T.,Sugimoto, H.,Owada, S.,Hough, M.A.,Worrall, J.A.R.,Owen, R.L.
Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide.
Iucrj, 12:582-594, 2025

PubMed Abstract: Time-resolved X-ray crystallography is undergoing a renaissance due to the development of serial crystallography at synchrotron and XFEL beamlines. Crucial to such experiments are efficient and effective methods for uniformly initiating time-dependent processes within microcrystals, such as ligand binding, enzymatic reactions or signalling. A widely applicable approach is the use of photocaged substrates, where the photocage is soaked into the crystal in advance and then activated using a laser pulse to provide uniform initiation of the reaction throughout the crystal. This work characterizes photocage release of nitric oxide and binding of this ligand to two heme protein systems, cytochrome c'-β and dye-decolourizing peroxidase B using a fixed target sample delivery system. Laser parameters for photoactivation are systematically explored, and time-resolved structures over timescales ranging from 100 µs to 1.4 s using synchrotron and XFEL beamlines are described. The effective use of this photocage for time-resolved crystallography is demonstrated and appropriate illumination conditions for such experiments are determined.

PubMed: 40843530
DOI: 10.1107/S2052252525006645

実験手法

X-RAY DIFFRACTION (1.8 Å)