9HPF

The NTD dimer and the interfacing LBD region of the AMPAR complex GluA3- TARP gamma2 in the desensitised state.

Summary for 9HPF

• Entry DOI: 10.2210/pdb9hpf/pdb
• EMDB information: 52328
• Descriptor: Isoform Flip of Glutamate receptor 3,Voltage-dependent calcium channel gamma-2 subunit, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: ampar, ion channels, neurotransmission, membrane protein
• Biological source: Rattus norvegicus (Norway rat); More
• Total number of polymer chains: 2
• Total formula weight: 265773.88

Authors

Pokharna, A.,Krieger, J.,Greger, I. (deposition date: 2024-12-12, release date: 2025-07-09, Last modification date: 2025-07-16)

Primary citation

Pokharna, A.,Stockwell, I.,Ivica, J.,Singh, B.,Schwab, J.,Vega-Gutierrez, C.,Herguedas, B.,Cais, O.,Krieger, J.M.,Greger, I.H.
Architecture, dynamics and biogenesis of GluA3 AMPA glutamate receptors.
Nature, 2025

PubMed Abstract: AMPA-type glutamate receptors (AMPARs) mediate the majority of excitatory neurotransmission in the brain. Assembled from combinations of four core subunits, GluA1-4, and ~20 auxiliary subunits, their molecular diversity tunes information transfer and storage in a brain circuit-specific manner. GluA3, a subtype strongly associated with disease, functions as both a fast transmitting Ca-permeable (CP) AMPAR at sensory synapses, and as a Ca-impermeable (CI) receptor at cortical synapses. Here, we present cryo-EM structures of the CP GluA3 homomer, which substantially diverge from other AMPARs. The GluA3 extracellular domain tiers (NTD and LBD) are closely coupled throughout gating states, creating previously unseen interfaces that impact signalling and harbour human disease mutations. Central to this architecture is a stacking interaction between two arginine residues (Arg163) in the NTD dimer interface, trapping a unique NTD dimer conformation that enables close contacts with the LBD. Rupture of the Arg163 stack not only alters the structure and dynamics of the GluA3 extracellular region, but also increases receptor trafficking, and the expression of GluA3 heteromers at the synapse. We further show that a mammalian-specific GluA3 trafficking checkpoint determines conformational stability of the LBD tier. Hence, specific design features define communication and biogenesis of GluA3, offering a framework to interrogate this disease-prone glutamate receptor.

PubMed: 40592473
DOI: 10.1038/s41586-025-09325-z

Experimental method

ELECTRON MICROSCOPY (3.77 Å)