9HMW

Structure of the Arabidopsis thaliana 80S ribosome OVAC mutant in complex with P- and E-site tRNAs and mRNA

これはPDB形式変換不可エントリーです。

9HMW の概要

• エントリーDOI: 10.2210/pdb9hmw/pdb
• EMDBエントリー: 52299
• 分子名称: Ribosomal RNA 5.8S, Small ribosomal subunit protein eS26y, Large ribosomal subunit protein uL6z/uL6y, ... (86 entities in total)
• 機能のキーワード: thermospermine, thaliana, ribosome, polyamines, ovac, 3-methyluridine
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 80
• 化学式量合計: 3195442.90

構造登録者

Faille, A.,Warren, A.J. (登録日: 2024-12-09, 公開日: 2026-02-04, 最終更新日: 2026-02-25)

主引用文献

Ko, D.,Ruonala, R.,Faille, A.,Hellmann, E.,Help, H.,Liu, H.,Nielsen, R.,Haakonsson, A.,De Diego, N.,Paatero, A.,Shcherbii, M.V.,Stefanowicz, K.,Cavar Zeljkovic, S.,Drud Lundager Rasmussen, T.,Novak, O.,Bodi, Z.,Eswaran, G.,Wybouw, B.,Bourdon, M.,Urbez, C.,Liu, X.,Salokas, K.,Ohman, T.,Waldie, T.,Toronen, P.,El-Showk, S.,Balcerowicz, M.,Besnard, F.,Liu, X.,Perkins, P.,Mazzoni-Putman, S.,Vainonen, J.P.,Sierla, M.,Frilander, M.J.,Mandrup, S.,Vernoux, T.,Ljung, K.,Ferrando, A.,Blazquez, M.A.,Holm, L.,Fray, R.,Varjosalo, M.,Leyser, O.,Paavilainen, V.O.,Mahonen, A.P.,Stepanova, A.,Alonso, J.,Heber, S.,Malinowski, R.,Kirpekar, F.,Warren, A.J.,Helariutta, Y.
Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate.
Science, 391:694-699, 2026

PubMed Abstract: Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In , the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR-OF-ACAULIS51 (SAC51) and SAC51-LIKEs (SACLs), which inhibit heterodimerization of the xylem development proteins LONESOME-HIGHWAY (LHW) and TARGET-OF-MONOPTEROS5. Here, we report a methyltransferase, OVERACHIEVER, that methylates the peptidyl transferase center of the 25 ribosomal RNA (rRNA). Residue mU2952 promotes functional tSpm binding to a specific site connecting the P-site transfer RNA (tRNA) with rRNA residues in the peptidyl transferase center. This interaction enhances the translation of SACLs but inhibits that of LHW. Our study uncovers the dependency between a conserved rRNA base methylation and a polyamine in orchestrating cell fate decisions, highlighting a role for the ribosome chemical landscape in translational regulation.

PubMed: 41678627
DOI: 10.1126/science.adx2867

実験手法

ELECTRON MICROSCOPY (2.25 Å)