9HDB

Sla1 SH3_3 domain (residues 355-414)

9HDB の概要

• エントリーDOI: 10.2210/pdb9hdb/pdb
• 分子名称: Actin cytoskeleton-regulatory complex protein SLA1 (2 entities in total)
• 機能のキーワード: sh3 domain, membrane trafficking, endocytosis, protein-protein interaction
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14524.73

構造登録者

Draper-Barr, G.,Defelipe, L.A.,Ruiz-Carillo, D.,Garcia-Alai, M.M. (登録日: 2024-11-12, 公開日: 2025-05-21, 最終更新日: 2025-07-16)

主引用文献

Draper-Barr, G.,Defelipe, L.A.,Ruiz-Carrillo, D.,Gustavsson, E.,Landau, M.,Garcia-Alai, M.
Sla2 is a core interaction hub for clathrin light chain and the Pan1/End3/Sla1 complex.
Structure, 33:1193-, 2025

PubMed Abstract: The interaction network of Sla2, a vital endocytic mid-coat adaptor protein, undergoes constant rearrangement. Sla2 serves as a scaffold linking the membrane to the actin cytoskeleton, with its role modulated by the clathrin light chain (CLC), which inhibits Sla2's function under certain conditions. We show that Sla2 has two independent binding sites for CLC: one previously described in homologs of fungi (Sla2) and metazoa (Hip1R), and a second found only in Fungi. We present the structural model of the Sla2 actin-binding domains in the context of regulatory structural domains by cryoelectron microscopy. We provide an interaction map of Sla2 and the regulatory proteins Sla1 and Pan1, predicted by AI modeling and confirmed by molecular biophysics techniques. Pan1 may compete with CLC for the conserved Sla2-binding site. These results enhance the mapping of crucial interactions at endocytic checkpoints and highlight the divergence between Metazoa and Fungi in this vital process.

PubMed: 40347949
DOI: 10.1016/j.str.2025.04.013

実験手法

X-RAY DIFFRACTION (1.491 Å)