9HBU

TiLV-NP tetramer (pseudo-C2) (local refinement around 2 TiLV-NPs)

9HBU の概要

• エントリーDOI: 10.2210/pdb9hbu/pdb
• EMDBエントリー: 52030
• 分子名称: Tilapia Lake Virus nucleoprotein (segment 4), 40-mer vRNA loop (2 entities in total)
• 機能のキーワード: viral protein, nucleoprotein, oligomer, rna binding protein
• 由来する生物種: Tilapia lake virus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125981.35

構造登録者

Arragain, B.,Cusack, S. (登録日: 2024-11-08, 公開日: 2025-03-12, 最終更新日: 2025-12-10)

主引用文献

Arragain, B.,Pelosse, M.,Huard, K.,Cusack, S.
Structure of the tilapia lake virus nucleoprotein bound to RNA.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Tilapia Lake virus (TiLV) belongs to the Amnoonviridae family within the Articulavirales order of segmented negative-strand RNA viruses and is highly diverged from more familiar orthomyxoviruses, such as influenza. The viral nucleoprotein (NP), a key component of the replication machinery, packages the viral genome into protective ribonucleoprotein particles. Here we describe the electron cryo-microscopy (cryo-EM) structure of TiLV-NP bound to RNA within in vitro reconstituted, small ring-like, pseudo-symmetrical oligomers. Although TiLV-NP is considerably smaller than its influenza counterpart and unrelated in sequence, it maintains the same topology and domain organisation. This comprises a head and body domain between which is a positively charged groove, where single-stranded RNA binds. In addition, an oligomerisation loop inserts into a hydrophobic pocket in the neighbouring NP, the flexible hinges of which allow variable orientation of adjacent NPs. Focused cryo-EM maps unambiguously define the 5' to 3' direction of the bound RNA, confirmed by double stranded, A-form RNA regions that extrude out from some of the NP-NP interfaces. This is the first fully resolved description of how single-stranded and stem-loop RNA binds to an articulaviral NP assembly. Superposition with orthomyxoviral NPs suggest that the mode of RNA binding is likely similar across the Articulavirales order.

PubMed: 39995042
DOI: 10.1093/nar/gkaf112

実験手法

ELECTRON MICROSCOPY (3.51 Å)