9H9R

Full gamma-tubulin ring complex composed of the Candida albicans gamma-tubulin small complex in complex with Spc72 CM1

これはPDB形式変換不可エントリーです。

9H9R の概要

• エントリーDOI: 10.2210/pdb9h9r/pdb
• 関連するPDBエントリー: 9H9P 9H9Q
• EMDBエントリー: 51971 51972
• 分子名称: Tubulin gamma chain, Spindle pole body component, Spc98p, ... (4 entities in total)
• 機能のキーワード: cytoskeleton, microtubule, microtubule nucleation, complex, template, cap, gamma-tubulin, gamma-tubulin ring complex, gamma-tubulin small complex, spc72, cm1, cm1 motif, gcp2, gcp3, spc97, spc98, candida, albicans, cell cycle, gamma-turc, gamma-tusc, yeast, fungi
• 由来する生物種: Candida albicans; 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 3175989.74

構造登録者

Vermeulen, B.J.A.,Pfeffer, S. (登録日: 2024-10-31, 公開日: 2025-01-15)

主引用文献

Zheng, A.,Vermeulen, B.J.A.,Wurtz, M.,Neuner, A.,Lubbehusen, N.,Mayer, M.P.,Schiebel, E.,Pfeffer, S.
Structural insights into the interplay between microtubule polymerases, gamma-tubulin complexes and their receptors.
Nat Commun, 16:402-402, 2025

PubMed Abstract: The γ-tubulin ring complex (γ-TuRC) is a structural template for controlled nucleation of microtubules from α/β-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the CM1-containing receptor protein Spc72 promotes γ-TuRC assembly from seven γ-tubulin small complexes (γ-TuSCs) and recruits the microtubule polymerase Stu2, yet their molecular interplay remains unclear. Here, we determine the cryo-EM structure of the Candida albicans cytoplasmic nucleation unit at 3.6 Å resolution, revealing how the γ-TuRC is assembled and conformationally primed for microtubule nucleation by the dimerised Spc72 CM1 motif. Two coiled-coil regions of Spc72 interact with the conserved C-terminal α-helix of Stu2 and thereby position the α/β-tubulin-binding TOG domains of Stu2 in the vicinity of the microtubule assembly site. Collectively, we reveal the function of CM1 motifs in γ-TuSC oligomerisation and the recruitment of microtubule polymerases to the γ-TuRC.

PubMed: 39757296
DOI: 10.1038/s41467-024-55778-7

実験手法

ELECTRON MICROSCOPY (8.2 Å)